Skip to Main content Skip to Navigation
Journal articles

Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008

Abstract : The geographical distribution and impact on animal and human health of both West Nile and Usutu viruses, two flaviviruses of the Japanese encephalitis complex, have been increasing during the past two decades. Both viruses circulate in Europe and Africa within a natural cycle between wild birds and mosquitoes, mainly from the Culex genus. We retrospectively analyzed sera from domestic and wild birds sampled in 2008 in two wetlands, namely the Inner Niger Delta, Mali, and the Lake Alaotra area, Madagascar. Sera were first tested using a commercial ID Screen West Nile Competition Multi-species ELISA kit. Then, positive sera and sera with insufficient volume for testing with ELISA were tested with a Microneutralization Test. In Mali, the observed seroprevalence in domestic birds was 28.5% [24.5; 32.8] (95%CI), 3.1 % [1.8; 5.2] (95%CI), 6.2% [3.4; 10.2] (95%CI) and 9.8 % [7.3; 12.8] (95%CI), for West Nile virus (WNV), Usutu virus (USUV), undetermined flavivirus, and WNV/USUV respectively. Regarding domestic birds of Madagascar, the observed seroprevalence was 4.4 % [2.1; 7.9](95%CI) for WNV, 0.9% [0.1; 3.1] (95%CI) for USUV, 1.3% [0.5; 2.8] (95%CI) for undetermined flavivirus, and null for WNV/USUV. Among the 150 wild birds sampled in Madagascar, two fulvous whistling-ducks (Dendrocygna bicolor) were positive for WNV and two for an undetermined flavivirus. One white-faced whistling-duck (Dendrocygna viduata) and one Hottentot teal (Spatula hottentota) were tested positive for USUV. African and European wetlands are linked by wild bird migrations. This first detection of USUV-as well as the confirmed circulation of WNV in domestic birds of two wetlands of Mali and Madagascar-emphasizes the need to improve the surveillance, knowledge of epidemiological patterns, and phylogenetic characteristics of flavivirus in Africa, particularly in areas prone to sustained, intense flavivirus transmission such as wetlands.
Complete list of metadatas

Cited literature [81 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02893795
Contributor : Hélène Lesur <>
Submitted on : Wednesday, July 8, 2020 - 3:25:08 PM
Last modification on : Thursday, July 9, 2020 - 6:17:27 PM

File

ij2020_erph_chevalier.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Véronique Chevalier, Maud Marsot, Sophie Molia, Harena Rasamoelina, René Rakotondravao, et al.. Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008. International Journal of Environmental Research and Public Health, MDPI, 2020, 17 (6), pp.1998. ⟨10.3390/ijerph17061998⟩. ⟨hal-02893795⟩

Share

Metrics

Record views

40

Files downloads

35