Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.

Philippe Blanchard 1, * Violaine Olivier 1 Anne-Laure Iscache 1 Olivier Celle 1 Frank Schurr 1 Perrine Lallemand 1 Magali Ribière 1
* Corresponding author
1 AFSSA - Agence Française de Sécurité Sanitaire des Aliments
Abstract : A new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates.
Keywords : Apis mellifera. Chronic bee paralysis virus (CBPV) RT-PCR diagnosis Genomic variability Apis mellifera
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Journal articles
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Submitted on : Tuesday, September 8, 2009 - 9:13:29 PM
Last modification on : Tuesday, October 23, 2018 - 9:54:02 PM
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Philippe Blanchard, Violaine Olivier, Anne-Laure Iscache, Olivier Celle, Frank Schurr, et al.. Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.. Journal of Invertebrate Pathology, Elsevier, 2008, 97 (2), pp.182-5. ⟨10.1016/j.jip.2007.07.003⟩. ⟨hal-00403564⟩

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