Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.

Philippe Blanchard 1, * Violaine Olivier 1 Anne-Laure Iscache 1 Olivier Celle 1 Frank Schurr 1 Perrine Lallemand 1 Magali Ribière 1
* Auteur correspondant
1 AFSSA - Agence Française de Sécurité Sanitaire des Aliments
Abstract : A new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates.
Keywords : Apis mellifera. Chronic bee paralysis virus (CBPV) RT-PCR diagnosis Genomic variability Apis mellifera
Type de document :
Article dans une revue
Journal of Invertebrate Pathology, Elsevier, 2008, 97 (2), pp.182-5. 〈10.1016/j.jip.2007.07.003〉
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Contributeur : Philippe Blanchard <>
Soumis le : mardi 8 septembre 2009 - 21:13:29
Dernière modification le : mercredi 9 septembre 2009 - 19:56:07
Document(s) archivé(s) le : mardi 15 juin 2010 - 19:52:57

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Philippe Blanchard, Violaine Olivier, Anne-Laure Iscache, Olivier Celle, Frank Schurr, et al.. Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.. Journal of Invertebrate Pathology, Elsevier, 2008, 97 (2), pp.182-5. 〈10.1016/j.jip.2007.07.003〉. 〈hal-00403564〉

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