Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.

Philippe Blanchard (1) , Violaine Olivier (1) , Anne-Laure Iscache (1) , Olivier Celle (1) , Frank Schurr (1) , Perrine Lallemand (1) , Magali Ribière (1)

1 AFSSA - Agence Française de Sécurité Sanitaire des Aliments

A new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates.

Mots clés: Apis mellifera.; Chronic bee paralysis virus (CBPV); RT-PCR diagnosis; Genomic variability; Apis mellifera

Domaines: Biologie moléculaire

Philippe Blanchard, Violaine Olivier, Anne-Laure Iscache, Olivier Celle, Frank Schurr, et al.. Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.. Journal of Invertebrate Pathology, 2008, 97 (2), pp.182-5. ⟨10.1016/j.jip.2007.07.003⟩. ⟨hal-00403564⟩