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Journal Articles Microbial Drug Resistance Year : 2012

Establishing streptomycin epidemiological cut-off values for Salmonella and Escherichia coli.

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Lourdes Garcia-Migura
  • Function : Author
Marianne Sunde
  • Function : Author
Susanne Karlsmose
  • Function : Author
Kees Veldman
  • Function : Author
Andreas Schroeter
  • Function : Author
Beatriz Guerra
  • Function : Author
Sophie A Granier
Alessia Franco
  • Function : Author
Stina Englund
  • Function : Author
Christopher Teale
  • Function : Author
Helmi Heiska
  • Function : Author
Lurdes Clemente
  • Function : Author
Patrick Boerlin
  • Function : Author
Miguel A Moreno
  • Function : Author
Danielle Daignault
  • Function : Author
Dik Mevius
  • Function : Author
Rene S Hendriksen
  • Function : Author
Frank M Aarestrup
  • Function : Author

Abstract

This study was conducted to elucidate the accuracy of the current streptomycin epidemiological cut-off value (ECOFF) for Escherichia coli and Salmonella spp. A total of 236 Salmonella enterica and 208 E. coli isolates exhibiting MICs between 4 and 32 mg/L were selected from 12 countries. Isolates were investigated by polymerase chain reaction for aadA, strA, and strB streptomycin resistance genes. Out of 236 Salmonella isolates, 32 (13.5%) yielded amplicons for aadA (n = 23), strA (n = 9), and strB (n = 11). None of the 60 Salmonella isolates exhibiting MIC 4 mg/L harbored resistance genes. Of the Salmonella isolates exhibiting MICs 8 mg/L, 16 mg/L, and 32 mg/L, 1.6%, 15%, and 39%, respectively, tested positive for one or more genes. For most monitoring programs, the streptomycin ECOFF for Salmonella is wild type (WT) ≤32 or ≤16 mg/L. A cut-off value of WT ≤32 mg/L would have misclassified 13.5% of the strains as belonging to the WT population, since this proportion of strains harbored resistance genes and exhibited MICs ≤32 mg/L. Out of 208 E. coli strains, 80 (38.5%) tested positive for aadA (n = 69), strA (n = 18), and strB (n = 31). Of the E. coli isolates exhibiting MICs of 4 mg/L, 8 mg/L, 16 mg/L, and 32 mg/L, 3.6%, 17.6%, 53%, and 82.3%, respectively, harbored any of the three genes. Based on the European Committee on Antimicrobial Susceptibility Testing guidelines (ECOFF ≤16 mg/L), 25% of the E. coli strains presenting MIC ≤16 mg/L would have been incorrectly categorized as belonging to the WT population. The authors recommend an ECOFF value of WT ≤16 mg/L for Salmonella and WT ≤8 mg/L for E. coli.

Dates and versions

hal-00692478 , version 1 (30-04-2012)

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Lourdes Garcia-Migura, Marianne Sunde, Susanne Karlsmose, Kees Veldman, Andreas Schroeter, et al.. Establishing streptomycin epidemiological cut-off values for Salmonella and Escherichia coli.. Microbial Drug Resistance, 2012, 18 (1), pp.88-93. ⟨10.1089/mdr.2011.0064⟩. ⟨hal-00692478⟩

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