HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Pseudorabies virus glycoprotein B can be used to carry foot and mouth disease antigens in DNA vaccination of pigs.

Abstract : To evaluate the feasibility of using pseudorabies virus (PrV) glycoprotein B (gB) as a carrier of foot and mouth disease virus (FMDV) antigens in DNA immunization, FMDV B- and T-cell epitopes were inserted either between the two B-cell epitopes of the N-term subunit of PrV-gB (BT-PrV-gB-N-term construct) or within the B-cell epitope of the C-term subunit of PrV-gB (BT-PrV-gB-C-term construct). Two animal experiments were performed, each with three injections of plasmids 2 weeks apart, followed by a booster inoculation of peptides corresponding to the FMDV epitopes. Control groups of pigs were injected with plasmids encoding either PrV-gB or FMDV-BT, or with empty-pcDNA3. The results of both assays were combined. Significant titers of FMDV neutralizing antibodies were detected after the peptides boost in groups injected with the BT-PrV-gB-C-term construct. Insignificant amounts were detected in groups injected with the BT-PrV-gB-N-term and FMDV-BT constructs. PBMCs from the BT-PrV-gB-N-term groups, isolated after the peptide boost injection, produced IFN-gamma and IL-4 mRNAs in vitro when stimulated with FMDV peptides. This was not observed with the other groups. These results imply that PrV-gB can be used to carry FMDV antigens in a DNA vaccine.
Document type :
Journal articles
Complete list of metadata

Cited literature [43 references]  Display  Hide  Download

https://hal-anses.archives-ouvertes.fr/hal-00404242
Contributor : Daniel Dory Connect in order to contact the contributor
Submitted on : Wednesday, July 15, 2009 - 5:37:37 PM
Last modification on : Thursday, April 7, 2022 - 10:10:09 AM
Long-term archiving on: : Tuesday, June 15, 2010 - 6:45:33 PM

File

Dory_D-AVR_2009-81-217-225-pre...
Files produced by the author(s)

Identifiers

Collections

Citation

Daniel Dory, Michelle Rémond, Véronique Béven, Roland Cariolet, Marija Backovic, et al.. Pseudorabies virus glycoprotein B can be used to carry foot and mouth disease antigens in DNA vaccination of pigs.. Antiviral Research, Elsevier Masson, 2009, 81 (3), pp.217-25. ⟨10.1016/j.antiviral.2008.11.005⟩. ⟨hal-00404242⟩

Share

Metrics

Record views

363

Files downloads

444