Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains. - Archive ouverte HAL Access content directly
Journal Articles Journal of Microbiological Methods Year : 2008

Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

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Abstract

The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.

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hal-00375588 , version 1 (15-04-2009)

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Benoît Doublet, Gregory Douard, Hayette Targant, Danièle Meunier, Jean-Yves Madec, et al.. Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.. Journal of Microbiological Methods, 2008, 75 (2), pp.359-61. ⟨10.1016/j.mimet.2008.06.010⟩. ⟨hal-00375588⟩

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