Skip to Main content Skip to Navigation
Journal articles

Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

Benoît Doublet 1, * Gregory Douard 1 Hayette Targant 2 Danièle Meunier 2 Jean-Yves Madec 2 Axel Cloeckaert 1
* Corresponding author
1 INRA
2 Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes
Abstract : The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.
keyword : XUBACTERIOLOGIE HAFSSA ANTIBIORESISTANCE
Document type :
Journal articles
Complete list of metadata
https://hal-anses.archives-ouvertes.fr/hal-00375588
Contributor : Valérie Cespedes <>
Submitted on : Wednesday, April 15, 2009 - 3:12:24 PM
Last modification on : Wednesday, March 10, 2021 - 3:33:48 AM

Identifiers

Collections

ANSES | INRA | INRAE

Citation

Benoît Doublet, Gregory Douard, Hayette Targant, Danièle Meunier, Jean-Yves Madec, et al.. Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.. Journal of Microbiological Methods, Elsevier, 2008, 75 (2), pp.359-61. ⟨10.1016/j.mimet.2008.06.010⟩. ⟨hal-00375588⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

369