Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

Benoît Doublet (1) , Gregory Douard (1) , Hayette Targant (2) , Danièle Meunier (2) , Jean-Yves Madec (2) , Axel Cloeckaert (1)

1 INRA
2 Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes

The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.

Keywords: XUBACTERIOLOGIE; HAFSSA; ANTIBIORESISTANCE

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology

Benoît Doublet, Gregory Douard, Hayette Targant, Danièle Meunier, Jean-Yves Madec, et al.. Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.. Journal of Microbiological Methods, 2008, 75 (2), pp.359-61. ⟨10.1016/j.mimet.2008.06.010⟩. ⟨hal-00375588⟩