Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

Abstract : The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.
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Journal of Microbiological Methods, Elsevier, 2008, 75 (2), pp.359-61. 〈10.1016/j.mimet.2008.06.010〉
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Contributeur : Valérie Cespedes <>
Soumis le : mercredi 15 avril 2009 - 15:12:24
Dernière modification le : mardi 23 octobre 2018 - 21:54:02

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Benoît Doublet, Gregory Douard, Hayette Targant, Danièle Meunier, Jean-Yves Madec, et al.. Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.. Journal of Microbiological Methods, Elsevier, 2008, 75 (2), pp.359-61. 〈10.1016/j.mimet.2008.06.010〉. 〈hal-00375588〉

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