Molecular differentiation of Mycoplasma gallisepticum and Mycoplasma imitans strains by pulsed-field gel electrophoresis and random amplified polymorphic DNA.

C. Marois 1 F. Dufour-Gesbert 1 I. Kempf 1
1 Laboratoire d'études et de recherches avicoles, porcines et piscicoles
Abstract : Pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) analysis were used to compare 21 Mycoplasma gallisepticum strains and five M. imitans strains. Each strain of M. gallisepticum typed by PFGE and RAPD methods was genetically quite unique and RAPD and PFGE fingerprinting enabled strain characterization. Relationships between the M. gallisepticum and M. imitans strains were established and dendrograms were drawn from PFGE and RAPD patterns. PFGE group A and RAPD group D were significantly associated with M. imitans strains (P < 0.05). Three M. imitans strains shared the same PFGE and RAPD patterns. The two M. gallisepticum vaccine strains had singular PFGE and RAPD patterns. Thus, PFGE and RAPD can be used to investigate disease outbreaks in vaccinated flocks or for epidemiological tracking. For M. gallisepticum, the RAPD and PFGE discriminatory powers were superior to 0.95 and the in vitro, in ovo and in vivo reproducibility of RAPD and PFGE was 100%. The RAPD drawback was the inconsistent band intensity complicating the interpretation of patterns, while the PFGE limit was its low typeability (86%). Thus, these two molecular typing methods seemed complementary for M. gallisepticum epidemiological studies.
Type de document :
Article dans une revue
Journal of Veterinary Medicine Series B, 2001, 48 (9), pp.695-703
Liste complète des métadonnées
https://hal-anses.archives-ouvertes.fr/hal-00366195
Contributeur : Isabelle Kempf <>
Soumis le : vendredi 6 mars 2009 - 10:22:33
Dernière modification le : mardi 14 avril 2009 - 17:42:26

Identifiants

  • HAL Id : hal-00366195, version 1
  • PUBMED : 11765805

Collections

ANSES

Citation

C. Marois, F. Dufour-Gesbert, I. Kempf. Molecular differentiation of Mycoplasma gallisepticum and Mycoplasma imitans strains by pulsed-field gel electrophoresis and random amplified polymorphic DNA.. Journal of Veterinary Medicine Series B, 2001, 48 (9), pp.695-703. 〈hal-00366195〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

57

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD