Draft genome sequence of a CTX-M-15-producing Escherichia coli ST345 from commercial chicken meat in Brazil

Tiago Casella (1) , Louise T Cerdeira (2) , Miriam R Fernandes (2) , Tiago A Souza (3) , Marisa Haenni (4) , Jean-Yves Madec (4) , Nilton Lincopan (5) , Mara C L Nogueira (6)

1 UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University
2 Faculdade de Ciências Farmacêuticas de Ribeirão Preto [São Paulo]
3 ICB/USP - Institute of Biomedical Sciences
4 AVB - Unité Antibiorésistance et Virulence Bactériennes
5 USP - Universidade de São Paulo = University of São Paulo
6 FAMERP - Faculdade de Medicina de São José do Rio Preto

Escherichia coli, the main host of the CTX-M-15 extended-spectrum b-lactamase (ESBL) enzyme, is widely distributed and exchanged between the environment, animals and humans. Therefore, identification of bla CTX-M-15-positive lineages in food has a significant impact on public health. In this regard, until the end of 1990s, ESBL-producing isolates were mainly associated with hospital-acquired infections, with a predominance of SHV-and TEM-type enzymes. In recent years, a new trend has been observed among ESBL-producers, where most isolates now harbour CTX-M-type, being further isolated from communityacquired infections. Nowadays, CTX-M-15 has been recognised as the most important ESBL variant, invading virtually all human and animal compartments, leading to a global pandemic. Thus, whilst the rapid emergence and dissemination of CTX-M-15 among E. coli isolates has generated a large genetic reservoir from which other members of the Enterobacteriaceae family can easily acquire this resistance gene, there are an increasing number of new reservoirs and transmission mechanisms that must be investigated. In this study, we present the draft genome sequence of a CTX-M-15-producing E. coli ST345 isolated from commercial chicken meat in Brazil. This draft genome can be used as a reference sequence for comparative analysis among CTX-M-15-producers.

Mots clés: Escherichia coli; Extended-spectrum b-lactamase; IncX1; Food; WGS; Brazil

Domaines: Bactériologie

Tiago Casella, Louise T Cerdeira, Miriam R Fernandes, Tiago A Souza, Marisa Haenni, et al.. Draft genome sequence of a CTX-M-15-producing Escherichia coli ST345 from commercial chicken meat in Brazil. Journal of Global Antimicrobial Resistance, 2017, 9, pp.124 - 125. ⟨10.1016/j.jgar.2017.04.002⟩. ⟨anses-04013271⟩