New reference genomes of Mycobacterium bovis adapted to French genotype diversity

Ciriac Charles (1) , Lorraine Michelet (1) , Fabien Vorimore (2) , Cyril Conde (3) , Thierry Cochard (3) , Franck Biet (3) , Maria Laura Boschiroli (1)

1 UZB - Unité Zoonoses Bactériennes
2 LSAl - Laboratoire de sécurité des aliments de Maisons-Alfort
3 UMR ISP - Infectiologie et Santé Publique

Bovine tuberculosis ( cattle outbreaks in France are present in specific regions and circulate in a multi host system that includes not only domestic but also wild animals. The transmission link between infected animals remains difficult to establish given that they are locally infected M bovis strains with identical genotypes (spoligotype MIRU VNTR). Whole genome SNP (single nucleotide polymorphisms) compared to appropriate reference genomes can precisely differentiate strains. However, the previous reference genome (AF 2122 97 belongs to the European 1 clonal complex mainly found in the British Isles but less in France or in other European countries 3 New reference genomes genetically close to French field strains, such as Mb 3601 that allowed us to describe the Eu 3 clonal complex, are required to perform precise field molecular epidemiological studies.

Domaines: Médecine vétérinaire et santé animale

Ciriac Charles, Lorraine Michelet, Fabien Vorimore, Cyril Conde, Thierry Cochard, et al.. New reference genomes of Mycobacterium bovis adapted to French genotype diversity. 7th International conference on Mycobacterium bovis, Jun 2022, Galway, Ireland. ⟨anses-03947361⟩