An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan - Archive ouverte HAL Access content directly
Journal Articles Microorganisms Year : 2020

An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan

, , , , , , , , , (1) , ,
1
Abdul Ghafar
Adil Khan
  • Function : Author
Alejandro Cabezas-Cruz
  • Function : Author
Charles Gauci
Sadaf Niaz
  • Function : Author
Sultan Ayaz
  • Function : Author
Lourdes Mateos-Hernández
  • Function : Author
Clemence Galon
  • Function : Author
Nasreen Nasreen
  • Function : Author
Robin Gasser
  • Function : Author
Abdul Jabbar

Abstract

This study investigated ticks and tick-borne microorganisms of small ruminants from five districts of the Federally Administered Tribal Area (FATA) of Pakistan. Morphological (n = 104) and molecular (n = 54) characterization of the ticks revealed the presence of six ixodid ticks: Rhipicephalus (Rh.) haemaphysaloides, Rh. microplus, Rh. turanicus, Haemaphysalis (Hs.) punctata, Hs. sulcata and Hyalomma anatolicum. Phylogenetic analyses of nucleotide sequence data for two mitochondrial (16S and cytochrome c oxidase 1) and one nuclear (second internal transcribed spacer) DNA regions provided strong support for the grouping of the six tick species identified in this study. Microfluidic real-time PCR, employing multiple pre-validated nuclear and mitochondrial genetic markers, detected 11 potential pathogens and endosymbionts in 72.2% of the ticks (n = 54) tested. Rickettsia (R.) massiliae was the most common pathogen found (42.6% of ticks) followed by Theileria spp. (33.3%), Anaplasma (A.) ovis and R. slovaca (25.9% each). Anaplasma centrale, A. marginale, Ehrlichia spp., R. aeschlimannii, R. conorii and endosymbionts (Francisella- and Coxiella-like) were detected at much lower rates (1.9–22.2%) in ticks. Ticks from goats (83.9%) carried significantly higher microorganisms than those from sheep (56.5%). This study demonstrates that ticks of small ruminants from the FATA are carrying multiple microorganisms of veterinary and medical health significance and provides the basis for future investigations of ticks and tick-borne diseases of animals and humans in this and neighboring regions.

Dates and versions

anses-03943038 , version 1 (17-01-2023)

Identifiers

Cite

Abdul Ghafar, Adil Khan, Alejandro Cabezas-Cruz, Charles Gauci, Sadaf Niaz, et al.. An Assessment of the Molecular Diversity of Ticks and Tick-Borne Microorganisms of Small Ruminants in Pakistan. Microorganisms, 2020, 8 (9), pp.1428. ⟨10.3390/microorganisms8091428⟩. ⟨anses-03943038⟩

Collections

ANSES ENVA INRAE
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More