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Screening of food raw materials for the presence of Shiga toxin-producing Escherichia coli O91:H21

Abstract : Aims: To provide information on the prevalence and detection, in foods, of Shiga toxin-producing Escherichia coli (STEC) O91:H21. Methods and Results: Seven hundred fifteen minced beef meats and 205 raw milk samples were analysed by stx-specific PCR-ELISA. Samples positive for stx were subsequently tested for the presence of wzy-O91, fliC-H21 and the adhesin-encoding gene saa. For minced meat, 16 (2AE2%) and 11 (1AE5%) samples were found positive for (stx, wzy-O91, fliC-H21) and (stx, wzy-O91, fliC-H21, saa) combinations, respectively. For raw milk, seven (3AE4%) samples were found positive for the (stx, wzy-O91, fliC-H21) combination but none of these contained saa. Two STEC O91:H21 saa-positive strains and three STEC O91 H21-and saa-negative strains were isolated by colony hybridization. Conclusions: A low prevalence of potentially pathogenic STEC O91:H21 in food products was found using a combination of PCR assays targeting stx, wzy-O91, fliC-H21 and saa. Significance and Impact of the Study: The PCR-based approach described here represents a valuable method for rapid screening of food samples contaminated by STEC O91:H21.
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https://hal-anses.archives-ouvertes.fr/anses-03858050
Contributor : Sylviane Derzelle Connect in order to contact the contributor
Submitted on : Monday, November 21, 2022 - 9:05:09 AM
Last modification on : Monday, November 21, 2022 - 9:05:09 AM

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J. Madic, C. Lecureuil, F. Dilasser, Sylviane Derzelle, E. Jamet, et al.. Screening of food raw materials for the presence of Shiga toxin-producing Escherichia coli O91:H21. Letters in Applied Microbiology, 2009, 48 (4), pp.447-451. ⟨10.1111/j.1472-765X.2008.02549.x⟩. ⟨anses-03858050⟩

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