Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass - Archive ouverte HAL Access content directly
Journal Articles Environment International Year : 2022

Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass

(1) , (2) , (3) , (4) , (5) , (5) , (1) , (1) , (1)
1
2
3
4
5

Abstract

Plastic pollution in marine ecosystems constitutes an important threat to marine life. For vertebrates, macro/microplastics can obstruct and/or transit into the airways and digestive tract whereas nanoplastics (NPs; < 1000 nm) have been observed in non-digestive tissues such as the liver and brain. Whether NPs cross the intestinal epithelium to gain access to the blood and internal organs remains controversial, however. Here, we show directly NP translocation across the intestinal barrier of a fish, the European seabass, Dicentrarchus labrax, ex vivo. The luminal side of median and distal segments of intestine were exposed to fluorescent polystyrene NPs (PS-NPs) of 50 nm diameter. PS-NPs that translocated to the serosal side were then detected quantitatively by fluorimetry, and qualitatively by scanning electron microscopy (SEM) and pyrolysis coupled to gas chromatography and high-resolution mass spectrometry (Py-GC-HRMS). Fluorescence intensity on the serosal side increased 15–90 min after PS-NP addition into the luminal side, suggesting that PS-NPs crossed the intestinal barrier; this was confirmed by both SEM and Py-GC-HRMS. This study thus evidenced conclusively that NPs beads translocate across the intestinal epithelium in this marine vertebrate.
Fichier principal
Vignette du fichier
Vagner_et_al_submitted_Env.Inter-final_V3_final.pdf (915.64 Ko) Télécharger le fichier
Vignette du fichier
1-s2.0-S0160412022002677-mmc1 (1).docx (3.99 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

anses-03695684 , version 1 (16-11-2022)

Licence

Attribution - NonCommercial - NoDerivatives - CC BY 4.0

Identifiers

Cite

Marie Vagner, Gaëlle G. Boudry, Lucie Courcot, Dorothée Vincent, Alexandre Dehaut, et al.. Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass. Environment International, 2022, 166, pp.107340. ⟨10.1016/j.envint.2022.107340⟩. ⟨anses-03695684⟩
34 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More