HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

M. Wasniewski 1 I. Almeida 2 A. Baur 3 T. Bedekovic 4 D. Boncea 5 L.B. Chaves 6 D. David 7 P. de Benedictis 8 M. Dobrostana 9 P. Giraud 10 P. Hostnik 11 I. Jaceviciene 12 S. Kenklies 13 M. König 14 K. Mähar 15 M. Mojzis 16 S. Moore 17 S. Mrenoski 18 T. Müller 19 E. Ngoepe 20 M. Nishimura 21 T. Nokireki 22 N. Pejovic 23 M. Smreczak 24 B. Strandbygaard 25 E. Wodak 26 F. Cliquet 1
1 LRFSN - Laboratoire de la rage et de la faune sauvage de Nancy
2 Laboratorio Nacional de Investigacao Veterinaria (LNIV)
3 Vet Med Labor GmbH
4 Croatian Veterinary Institute Laboratory for Rabies/Virology
5 Institute for Diagnosis and Animal Health
6 Laboratório de Diagnóstico da Raiva, Instituto Pasteur
7 Kimron Veterinary Institute Rabies Laboratory
8 IZSVe - Istituto Zooprofilattico Sperimentale delle Venezie
9 BIOR - Institute of Food Safety, Animal Health and Environment
10 Laboratoire Départemental d'Analyses du Pas-de-Calais
11 SVA - National Veterinary Institute [Uppsala]
12 National Food and Veterinary Risk Assessment Institute of Lithuania
13 Landesamt für Verbraucherschutz Sachsen-Anhalt, Fachbereich Veterinärmedizin
14 JLU - Justus-Liebig-Universität Gießen = Justus Liebig University
15 Estonian Veterinary and Food Laboratory
16 State Veterinary Institute Zvolen
17 Kansas State University
18 UKIM - Ss. Cyril and Methodius University in Skopje
19 FLI - Friedrich-Loeffler-Institut
20 Agricultural Research Council-Onderstepoort Veterinary Institute
21 Research Institute for Animal Science In Biochemistry and Toxicology
22 Finnish Food safety Authority
23 Diagnostic Veterinary Laboratory Montenegro
24 NVRI - National Veterinary Research Institute [Pulawy, Pologne]
25 DTU - Danmarks Tekniske Universitet
26 AGES - Institute for Veterinary Disease Control Mödling
Abstract : The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7%, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2% and 97.5%, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95%. The coefficients of concordance obtained by laboratories ranged from 87.2% to 100%. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.
Keywords : Collaborative study ELISA Oral vaccination campaigns Rabies Rabies antibodies
Document type :
Journal articles
Complete list of metadata
https://hal-anses.archives-ouvertes.fr/anses-03433401
Contributor : Sophie Marchal-Mauer Connect in order to contact the contributor
Submitted on : Wednesday, November 17, 2021 - 5:09:38 PM
Last modification on : Monday, May 2, 2022 - 11:10:01 AM

Identifiers

Collections

ANSES

Citation

M. Wasniewski, I. Almeida, A. Baur, T. Bedekovic, D. Boncea, et al.. First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe. Journal of Virological Methods, Elsevier, 2016, 238, pp.77-85. ⟨10.1016/j.jviromet.2016.10.006⟩. ⟨anses-03433401⟩

Export

BibTeX TEI DC DCterms EndNote Datacite

Share

Metrics

Record views

9