Skip to Main content Skip to Navigation
Journal articles

First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

M. Wasniewski 1 I. Almeida 2 A. Baur 3 T. Bedekovic 4 D. Boncea 5 L.B. Chaves 6 D. David 7 P. de Benedictis 8 M. Dobrostana 9 P. Giraud 10 P. Hostnik 11 I. Jaceviciene 12 S. Kenklies 13 M. König 14 K. Mähar 15 M. Mojzis 16 S. Moore 17 S. Mrenoski 18 T. Müller 19 E. Ngoepe 20 M. Nishimura 21 T. Nokireki 22 N. Pejovic 23 M. Smreczak 24 B. Strandbygaard 25 E. Wodak 26 F. Cliquet 1
Abstract : The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7%, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2% and 97.5%, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95%. The coefficients of concordance obtained by laboratories ranged from 87.2% to 100%. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.
Complete list of metadata

https://hal-anses.archives-ouvertes.fr/anses-03433401
Contributor : Sophie Marchal-Mauer Connect in order to contact the contributor
Submitted on : Wednesday, November 17, 2021 - 5:09:38 PM
Last modification on : Wednesday, November 24, 2021 - 10:14:01 AM

Identifiers

Collections

Citation

M. Wasniewski, I. Almeida, A. Baur, T. Bedekovic, D. Boncea, et al.. First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe. Journal of Virological Methods, Elsevier, 2016, 238, pp.77-85. ⟨10.1016/j.jviromet.2016.10.006⟩. ⟨anses-03433401⟩

Share

Metrics

Record views

4