Longitudinal survey of two serotine bat (Eptesicus serotinus) maternity colonies exposed to EBLV-1 (European Bat Lyssavirus type 1): Assessment of survival and serological status variations using capture-recapture models

Emmanuelle Robardet (1) , Christophe Borel (2) , Marie Moinet (1) , Dorothée Jouan (2) , Marine Wasniewski (1) , Jacques Barrat (1) , Franck Boué (1) , Elodie Monchâtre-Leroy (1) , Alexandre Servat (1) , Olivier Gimenez (3) , Florence Cliquet (1) , Evelyne Picard-Meyer (1)

1 LRFSN - Laboratoire de la rage et de la faune sauvage de Nancy
2 CPEPESC Lorraine - Commission de Protection des Eaux, du Patrimoine, de l'Environnement, du Sous-sol et des Chiroptères de Lorraine
3 CEFE - Centre d’Ecologie Fonctionnelle et Evolutive

This study describes two longitudinal serological surveys of European Bat Lyssavirus type 1 (EBLV-1) antibodies in serotine bat (Eptesicus serotinus) maternity colonies located in the NorthEast of France. This species is currently considered as the main EBLV-1 reservoir. Multievent capture-recapture models were used to determine the factors influencing bat rabies transmission as this method accounts for imperfect detection and uncertainty in disease states. Considering the period of study, analyses revealed that survival and recapture probabilities were not affected by the serological status of individuals, confirming the capacity of bats to be exposed to lyssaviruses without dying. Five bats have been found with EBLV-1 RNA in the saliva at the start of the study, suggesting they were caught during virus excretion period. Among these bats, one was interestingly recaptured one year later and harbored a seropositive status. Along the survey, some others bats have been observed to both seroconvert (i.e. move from a negative to a positive serological status) and serorevert (i.e. move from a positive to a negative serological status). Peak of seroprevalence reached 34% and 70% in site A and B respectively. On one of the 2 sites, global decrease of seroprevalence was observed all along the study period nuanced by oscillation intervals of approximately 2-3 years supporting the oscillation infection dynamics hypothesized during a previous EBLV-1 study in a Myotis myotis colony. Seroprevalence were affected by significantly higher seroprevalence in summer than in spring. The maximum time observed between successive positive serological statuses of a bat demonstrated the potential

Mots clés: Bats; Animal sociality; Serology; Fruit bats; Lyssavirus; Spring; Saliva; Antibodies

Domaines: Médecine vétérinaire et santé animale; Santé publique et épidémiologie

Emmanuelle Robardet, Christophe Borel, Marie Moinet, Dorothée Jouan, Marine Wasniewski, et al.. Longitudinal survey of two serotine bat (Eptesicus serotinus) maternity colonies exposed to EBLV-1 (European Bat Lyssavirus type 1): Assessment of survival and serological status variations using capture-recapture models. PLoS Neglected Tropical Diseases, 2017, 11 (11), pp.e0006048. ⟨10.1371/journal.pntd.0006048⟩. ⟨anses-03429219⟩