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Quantification of 4-hydroxy-2-nonenal-protein adducts in in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

Abstract : Hydroxyalkenals are lipid oxidation end-products resulting from the oxidation of polyunsaturated fatty acids (PUFA). This study aimed at quantifying the production of 4-hydroxy-2-nonenal-protein adducts (HNE-P) via Michael addition from n-6 PUFA oxidation in the gastric digesta of mini-pigs after the consumption of meat-based meals with different plant antioxidant contents. Using the accuracy profile procedure, we validated an extraction protocol for the quantification of HNE-P by GC-MS/MS in gastric contents. The formation of HNE-P in the gastric compartment was observed for the first time, with concentrations ranging from less than 0.52 to 1.33 nmol HNE-P / 500 mg digesta. Nevertheless, most gastric HNE-P levels were below the limit of quantification of 0.52 nmol HNE-P / 500 mg digesta. In this animal study, the protective effect of plant antioxidant sources on HNE-P formation was not evidenced contrasting with results using TBARS as markers.
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https://hal.archives-ouvertes.fr/hal-01901450
Contributor : Archive Ouverte Prodinra <>
Submitted on : Monday, October 22, 2018 - 8:11:16 PM
Last modification on : Thursday, May 28, 2020 - 11:34:15 AM

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Mylène Delosière, Veronique Sante-Lhoutellier, Céline Chantelauze, Denys Durand, Agnes Thomas, et al.. Quantification of 4-hydroxy-2-nonenal-protein adducts in in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation. Food and Function, Cambridge : Royal Society of Chemistry, 2016, 7 (8), pp.3497-3504. ⟨10.1039/C6FO00413J⟩. ⟨hal-01901450⟩

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