Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

Abstract : Hydroxyalkenals are lipid oxidation end-products resulting from the oxidation of polyunsaturated fatty acids (PUFA). This study aimed at quantifying the production of 4-hydroxy-2-nonenal-protein adducts (HNE-P) via Michael addition from n-6 PUFA oxidation in the gastric digesta of mini-pigs after the consumption of meat-based meals with different plant antioxidant contents. Using the accuracy profile procedure, we validated an extraction protocol for the quantification of HNE-P by GC-MS/MS in gastric contents. The formation of HNE-P in the gastric compartment was observed for the first time, with concentrations ranging from less than 0.52 to 1.33 nmol HNE-P per 500 mg digesta. Nevertheless, most gastric HNE-P levels were below the limit of quantification of 0.52 nmol HNE-P per 500 mg digesta. In this animal study, the protective effect of plant antioxidant sources on HNE-P formation was not evidenced contrasting with the results using TBARS as markers.
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https://hal-anses.archives-ouvertes.fr/anses-01348274
Contributor : Emmanuelle Chiffoleau <>
Submitted on : Friday, July 22, 2016 - 4:18:25 PM
Last modification on : Wednesday, July 3, 2019 - 1:00:05 PM

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Mylène Delosière, Veronique Sante-Lhoutellier, Céline Chantelauze, D. Durand, Agnès Thomas, et al.. Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation. Food & Function, 2016, ⟨http://pubs.rsc.org/en/Content/ArticleLanding/2016/FO/C6FO00413J#!divAbstract⟩. ⟨10.1039/c6fo00413j⟩. ⟨anses-01348274⟩

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