Modulation of chromatin remodelling induced by the freshwater cyanotoxin cylindrospermopsin in human intestinal caco-2 cells.

Abstract : Cylindrospermopsin (CYN) is a cyanotoxin that has been recognised as an emerging potential public health risk. Although CYN toxicity has been demonstrated, the mechanisms involved have not been fully characterised. To identify some key pathways related to this toxicity, we studied the transcriptomic profile of human intestinal Caco-2 cells exposed to a sub-toxic concentration of CYN (1.6 µM for 24hrs) using a non-targeted approach. CYN was shown to modulate different biological functions which were related to growth arrest (with down-regulation of cdkn1a and uhrf1 genes), and DNA recombination and repair (with up-regulation of aptx and pms2 genes). Our main results reported an increased expression of some histone-modifying enzymes (histone acetyl and methyltransferases MYST1, KAT5 and EHMT2) involved in chromatin remodelling, which is essential for initiating transcription. We also detected greater levels of acetylated histone H2A (Lys5) and dimethylated histone H3 (Lys4), two products of these enzymes. In conclusion, CYN overexpressed proteins involved in DNA damage repair and transcription, including modifications of nucleosomal histones. Our results highlighted some new cell processes induced by CYN.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2014, 9 (6), pp.e99121. 〈10.1371/journal.pone.0099121〉
Liste complète des métadonnées

Littérature citée [52 références]  Voir  Masquer  Télécharger

https://hal-anses.archives-ouvertes.fr/hal-01025934
Contributeur : Emmanuelle Chiffoleau <>
Soumis le : vendredi 18 juillet 2014 - 17:28:30
Dernière modification le : vendredi 3 août 2018 - 16:10:02
Document(s) archivé(s) le : lundi 24 novembre 2014 - 20:06:14

Fichier

huguet2014PolsOne.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Antoine Huguet, Aurélie Hatton, Romain Villot, Hélène Quenault, Yannick Blanchard, et al.. Modulation of chromatin remodelling induced by the freshwater cyanotoxin cylindrospermopsin in human intestinal caco-2 cells.. PLoS ONE, Public Library of Science, 2014, 9 (6), pp.e99121. 〈10.1371/journal.pone.0099121〉. 〈hal-01025934〉

Partager

Métriques

Consultations de la notice

171

Téléchargements de fichiers

161