Comparison of the virucidal efficiency of peracetic acid, potassium monopersulfate and sodium hypochlorite on hepatitis A and enteric cytopathogenic bovine orphan virus.

Hélène Martin 1, * Christophe Soumet 1 Rachelle Fresnel 1 T. Morin S. Lamaudière A. L. Le Sauvage Karine Deleurme 1 Pierre Maris 1
* Corresponding author
1 ANSES - ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
Abstract : AIMS: The virucidal activity of peroxy-products was evaluated and compared with sodium hypochlorite using the EN 14675 European suspension test and a surface test developed in our laboratory. The classical approach on infectivity of viruses was complemented with a prospective approach on virus genomes. METHODS AND RESULTS: Both infectivity tests were adapted and/or developed to determine the activity of disinfectants against reference bovine enterovirus type 1 [enteric cytopathogenic bovine orphan virus (ECBO)] and resistant hepatitis A virus (HAV) in conditions simulating practical use. Similar concentrations of active chlorine were virucidal against both viruses, either at 0*062% using the suspension test or at 0*50-1% using the surface test. However, for potassium monopersulfate and peracetic acid products, concentrations of approximately three times (3%) to 72 times (9%) higher were necessary against HAV than ECBO when determined with the suspension test. With the surface test, 4-8% peroxy-products were virucidal against HAV, either 16 times more peroxy-products concentrations than against ECBO. No significant impact on the targeted area of the viral genome measured by real-time RT-PCRs was obtained for ECBO and HAV suspensions treated with disinfectants, even with doses higher than the minimal virucidal concentrations. CONCLUSIONS: Sodium hypochlorite, but not peroxy-products, had similar activity against ECBO and HAV. No relation could be established between infectivity tests and genome destruction. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first comparative study that investigates with novel suspension and surface tests the reduction of infectivity and genome destruction of two resistant viruses by peroxy-compounds. The results and conclusions collected with European standards are discussed.
Keywords : virucidal activity virus disinfection disinfectant peracetic acid dosium hypochlorite enteric cytopathogenic bovine orphan virus
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Journal articles
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Submitted on : Friday, October 4, 2013 - 4:11:31 PM
Last modification on : Tuesday, February 19, 2019 - 3:14:07 PM

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Hélène Martin, Christophe Soumet, Rachelle Fresnel, T. Morin, S. Lamaudière, et al.. Comparison of the virucidal efficiency of peracetic acid, potassium monopersulfate and sodium hypochlorite on hepatitis A and enteric cytopathogenic bovine orphan virus.. Journal of Applied Microbiology, Wiley, 2013, 115 (4), pp.955-68. ⟨10.1111/jam.12297⟩. ⟨hal-00870004⟩

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