Cytotoxicity, Fractionation and Dereplication of Extracts of the Dinoflagellate Vulcanodinium rugosum, a Producer of Pinnatoxin G.

Abstract : Pinnatoxin G (PnTX-G) is a marine toxin belonging to the class of cyclic imines and produced by the dinoflagellate Vulcanodinium rugosum. In spite of its strong toxicity to mice, leading to the classification of pinnatoxins into the class of "fast-acting toxins", its hazard for human health has never been demonstrated. In this study, crude extracts of V. rugosum exhibited significant cytotoxicity against Neuro2A and KB cells. IC50 values of 0.38 µg mL-1 and 0.19 µg mL-1 were estimated on Neuro2A cells after only 24 h of incubation and on KB cells after 72 h of incubation, respectively. In the case of Caco-2 cells 48 h after exposure, the crude extract of V. rugosum induced cell cycle arrest accompanied by a dramatic increase in double strand DNA breaks, although only 40% cytotoxicity was observed at the highest concentration tested (5 µg mL-1). However, PnTX-G was not a potent cytotoxic compound as no reduction of the cell viability was observed on the different cell lines. Moreover, no effects on the cell cycle or DNA damage were observed following treatment of undifferentiated Caco-2 cells with PnTX-G. The crude extract of V. rugosum was thus partially purified using liquid-liquid partitioning and SPE clean-up. In vitro assays revealed strong activity of some fractions containing no PnTX-G. The crude extract and the most potent fraction were evaluated using full scan and tandem high resolution mass spectrometry. The dereplication revealed the presence of a major compound that could be putatively annotated as nakijiquinone A, N-carboxy-methyl-smenospongine or stachybotrin A, using the MarinLit™ database. Further investigations will be necessary to confirm the identity of the compounds responsible for the cytotoxicity and genotoxicity of the extracts of V. rugosum.
Type de document :
Article dans une revue
Marine drugs, MDPI, 2013, 11 (9), pp.3350-71. 〈10.3390/md11093350〉
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

https://hal-anses.archives-ouvertes.fr/hal-00862461
Contributeur : Emmanuelle Chiffoleau <>
Soumis le : lundi 16 septembre 2013 - 17:01:01
Dernière modification le : vendredi 3 février 2017 - 01:01:31
Document(s) archivé(s) le : jeudi 6 avril 2017 - 21:01:22

Fichier

geiger2013.pdf
Accord explicite pour ce dépôt

Identifiants

Collections

Citation

Marie Geiger, Gwenaëlle Desanglois, Kevin Hogeveen, Valérie Fessard, Thomas Leprêtre, et al.. Cytotoxicity, Fractionation and Dereplication of Extracts of the Dinoflagellate Vulcanodinium rugosum, a Producer of Pinnatoxin G.. Marine drugs, MDPI, 2013, 11 (9), pp.3350-71. 〈10.3390/md11093350〉. 〈hal-00862461〉

Partager

Métriques

Consultations de la notice

293

Téléchargements de fichiers

325