Comparative Cytotoxicity, Oxidative Stress, and Cytokine Secretion Induced by Two Cyanotoxin Variants, Microcystin LR and RR, in Human Intestinal Caco-2 Cells.

Antoine Huguet 1, * Jérôme Henri 1 Mélanie Petitpas 1 Kevin Hogeveen 1 Valérie Fessard 1
* Corresponding author
1 Unité de Toxicologie des Contaminants
Laboratoire de Fougères - ANSES
Abstract : While MC-LR and MC-RR share significant structural similarity, MC-RR is less cytotoxic than MC-LR. In the current study, we have compared the effects of MC-LR and MC-RR in Caco-2 cells by evaluating cytotoxicity, oxidative stress (reactive oxygen species production), and the cellular proinflammatory response (IL-6 and IL-8 production). Following treatment with 100 µM microcystins (MC), cytotoxicity was twoxs-fold greater with MC-LR as compared to MC-RR after 24 h exposure. Whereas the reactive oxygen species production and IL-6 secretion were similar following a 24-h treatment with either MC, 100 µM MC-LR induced a five-fold greater IL-8 secretion when compared to MC-RR. Our study has demonstrated that, although both MC-LR and MC-RR induced some cytotoxicity in human intestinal cells, a major difference in IL-8 production was observed between the two variants.
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Journal articles
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Submitted on : Thursday, April 18, 2013 - 1:40:47 PM
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Antoine Huguet, Jérôme Henri, Mélanie Petitpas, Kevin Hogeveen, Valérie Fessard. Comparative Cytotoxicity, Oxidative Stress, and Cytokine Secretion Induced by Two Cyanotoxin Variants, Microcystin LR and RR, in Human Intestinal Caco-2 Cells.. Journal of Biochemical and Molecular Toxicology, Wiley, 2013, epub ahead of print. ⟨10.1002/jbt.21482⟩. ⟨hal-00815224⟩

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