Morphological cell transformation of Syrian hamster embryo (SHE) cells by the cyanotoxin, cylindrospermopsin. - Archive ouverte HAL Access content directly
Journal Articles Toxicon Year : 2010

Morphological cell transformation of Syrian hamster embryo (SHE) cells by the cyanotoxin, cylindrospermopsin.

Valérie Fessard
  • Function : Author
  • PersonId : 881770
C. Rast
  • Function : Author
A. R. Humpage
  • Function : Author
P. Vasseur

Abstract

Cylindrospermopsin (CYN) is a cyanotoxin which has been implicated in human intoxication and animal mortality. Genotoxic activity of this hepatotoxin is known but its carcinogenic activity remains to be elucidated. In this work, CYN was assessed for its cell-transforming activity using the Syrian hamster embryo (SHE) cell transformation assay. This in vitro assay is used to evaluate the carcinogenic potential of chemical, physical and biological agents in SHE cells, which are primary, normal, diploid, genetically stable and capable of metabolic activation. We demonstrated that CYN induced a significant increase in morphological cell transformation in SHE cells following a 7-day continuous treatment in the range of non-cytotoxic concentrations 1 x 10(-7)-1 x 10(-2) ng/mL.

Dates and versions

hal-00533772 , version 1 (08-11-2010)

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Marie-Aline Maire, E. Bazin, Valérie Fessard, C. Rast, A. R. Humpage, et al.. Morphological cell transformation of Syrian hamster embryo (SHE) cells by the cyanotoxin, cylindrospermopsin.. Toxicon, 2010, 55 (7), pp.1317-22. ⟨10.1016/j.toxicon.2010.01.025⟩. ⟨hal-00533772⟩
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