Development of serological proteome analysis of mastitis by Staphylococcus aureus in ewes. - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Accéder directement au contenu
Article Dans Une Revue Journal of Microbiological Methods Année : 2009

Development of serological proteome analysis of mastitis by Staphylococcus aureus in ewes.

Résumé

Staphylococcus aureus is a major agent of mastitis in ruminants worldwide. So far, efficient measures for its prophylaxis (including vaccination) have proven to be unsuccessful and there is a need for a better understanding of the host response to udder infection by S. aureus. Serological proteome analysis (SERPA) is a promising technique that can be used to identify S. aureus immuno-dominant determinants providing that bacterial culture conditions used to grow S. aureus strains for protein sample preparation mimic the context of mastitis. A S. aureus strain was used in experimental mastitis to generate sheep serum used to determine the best growth conditions for SERPA. Sera collected in the field from different ewes suffering from mastitis by S. aureus were used to confirm experimental observations. Three different culture media (BHI, whey and iron-depleted RPMI) were tested. The influence of aeration and growth phase on protein production was also evaluated by immuno-detection of protein samples prepared from cultures grown in different conditions and obtained from different culture fractions (supernatant, cell wall, and total lysates). Our results showed that culturing in iron-depleted RPMI with (secreted proteins, prepared from stationary phase) or without aeration (cell wall proteins, prepared from early stationary phase, and total proteins, prepared from exponential phase) is the condition that best mimics growth in vivo during mastitis and this in vitro growth condition is to be used henceforth in experiments involving SERPA.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
Development_of_serological_proteome_J._of_Microbiological_Methods_2009.pdf (668.34 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-00422155 , version 1 (06-10-2009)

Identifiants

Citer

C. Le Maréchal, Gwénaël Jan, Sergine Even, J. A. Mcculloch, V. Azevedo, et al.. Development of serological proteome analysis of mastitis by Staphylococcus aureus in ewes.. Journal of Microbiological Methods, 2009, 79 (1), pp.131-6. ⟨10.1016/j.mimet.2009.08.017⟩. ⟨hal-00422155⟩
305 Consultations
248 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More