Comparative analysis of both genomic segments of betanodaviruses isolated from epizootic outbreaks in farmed fish species provides evidence for genetic reassortment.

Jose G Olveira 1 Sandra Souto 1 Carlos P Dopazo 1 Richard Thiéry 2 Juan L Barja 1 Isabel Bandin 1, *
* Auteur correspondant
1 Instituto de Acuicultura
2 Laboratoire d'études et de recherches sur les petits ruminants et les abeilles
Abstract : Sequencing of the full coding region of both genomic segments of seven betanodavirus strains isolated from different farmed species in Spain and Portugal revealed that six of them were reassortants, exhibiting a RGNNV RNA1 and a SJNNV RNA2. Analysis of sequences of reassortant strains at both genomic and protein level revealed the existence of differences with type strains of both genotypes. These differences were higher in the polymerase sequence, which is remarkable because viral structural proteins generally diverge more rapidly than non-structural proteins. Changes in two amino acids observed in the SJNNV capsid protein might be involved in the colonization of new host species by these reassortant strains. In addition, a more extensive phylogenetic analysis, including partial sequences of both RNA segments of other 16 Iberian nodavirus, confirmed the existence of reassortment between RGNNV and SJNNV.
Type de document :
Article dans une revue
Journal of General Virology, Microbiology Society, 2009, epub ahead of print. 〈10.1099/vir.0.013912-0〉
Liste complète des métadonnées
https://hal-anses.archives-ouvertes.fr/hal-00418441
Contributeur : Françoise Bautey <>
Soumis le : vendredi 18 septembre 2009 - 15:41:32
Dernière modification le : mardi 23 octobre 2018 - 21:54:02

Lien texte intégral

openaccess

Identifiants

Collections

ANSES

Citation

Jose G Olveira, Sandra Souto, Carlos P Dopazo, Richard Thiéry, Juan L Barja, et al.. Comparative analysis of both genomic segments of betanodaviruses isolated from epizootic outbreaks in farmed fish species provides evidence for genetic reassortment.. Journal of General Virology, Microbiology Society, 2009, epub ahead of print. 〈10.1099/vir.0.013912-0〉. 〈hal-00418441〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

143

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD