Characterization of Staphylococcus aureus isolates recovered from dairy sheep farms (agr group, adherence, slime, resistance to antibiotics)

Eric Vautor (1) , Hélène Carsenti-Dellamonica (2) , M. Sabah (2) , Géraldine Mancini (2) , Michel Pépin (1) , Pierre Dellamonica (2)

1 UMR ERRC - Elevage des ruminants en régions chaudes
2 CHU Nice

Staphylococcus aureus is one of the major causes of dairy sheep mastitis. The S. aureus agr locus (accessory gene regulator) regulates the production of most staphylococcal exoproteins, including exoenzymes, toxins, surface proteins, and other virulence factors. S. aureus have four agr groups (alleles) determined by PCR. In this study, 46 S. aureus isolates, recovered in south-east of France, were also characterized by their properties of adherence to smooth surfaces, slime production and resistance to 10 antibiotics. For 46 S. aureus associated with dairy sheep mastitis (subclinical mastitis, clinical mastitis, environment of dairy sheep farm), 80% (37/46) belonged to agr group 3, 39% (18/46) were adherent (adherent, strongly adherent or with maximal adherence). For the same isolates, 26% (12/46) were slime producers (moderate or strong producers). All the 46 isolates were susceptible to oxacillin, except for two isolates including two sheep subclinical mastitis isolates. The dairy sheep subclinical mastitis isolates were for 79% (22/28), susceptible to nine other antibiotics tested.

Keywords: Adherence; Antibiotics; Dairy sheep mastitis; Slime; Staphylococcus aureus; Accessory gene regulator (agr)

Domains: Bacteriology

Eric Vautor, Hélène Carsenti-Dellamonica, M. Sabah, Géraldine Mancini, Michel Pépin, et al.. Characterization of Staphylococcus aureus isolates recovered from dairy sheep farms (agr group, adherence, slime, resistance to antibiotics). Small Ruminant Research, 2006, 72 (2-3), pp.197-199. ⟨10.1016/j.smallrumres.2006.08.011⟩. ⟨hal-00409252⟩