In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration.

Julien Gaudin 1 Ludovic Le Hegarat 1 Fabrice Nesslany Daniel Marzin Valérie Fessard 1
1 Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants
Abstract : Microcystin-LR (MC-LR) is a toxin produced by freshwater cyanobacteria and is a potential threat to human health. MC-LR has been shown to be both a specific inhibitor of serine/threonine protein phosphatases PP1 and PP2A and a potent tumor promoter in rat liver. However, the genotoxic potential of MCs remains unclear. In this article, we investigated the ability of MC-LR to induce DNA damage on rat hepatocytes following intravenous (iv) administration by using two in vivo genotoxicity assays: the unscheduled DNA synthesis (UDS) and the comet assays. The UDS assay measures DNA synthesis induced from the excision repair of DNA damaged regions and the comet assay is a very sensitive technique for detecting various forms of DNA damage. After an exposure time of 2-4 h or 12-16 h and a dose ranging from 12.5 to 50 microg/kg bw, no DNA damage could be observed in both assays on rat hepatocytes following iv administration. These findings have been discussed and compared with recently published genotoxic results obtained in other organs from mice after oral and intraperitoneal treatments to better understand the mechanism of action of this toxin in relation with its cancerogenicity potential.
Keywords : Comet assay Genotoxicity Microcystin-LR UDS assay
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Journal articles
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Submitted on : Monday, July 20, 2009 - 3:14:46 PM
Last modification on : Tuesday, October 23, 2018 - 9:54:02 PM

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Julien Gaudin, Ludovic Le Hegarat, Fabrice Nesslany, Daniel Marzin, Valérie Fessard. In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration.. Environmental Toxicology, Wiley, 2009, 24 (2), pp.200-9. ⟨10.1002/tox.20417⟩. ⟨hal-00405465⟩

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