In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration.

Julien Gaudin 1 Ludovic Le Hegarat 1 Fabrice Nesslany Daniel Marzin Valérie Fessard 1
1 Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants
Abstract : Microcystin-LR (MC-LR) is a toxin produced by freshwater cyanobacteria and is a potential threat to human health. MC-LR has been shown to be both a specific inhibitor of serine/threonine protein phosphatases PP1 and PP2A and a potent tumor promoter in rat liver. However, the genotoxic potential of MCs remains unclear. In this article, we investigated the ability of MC-LR to induce DNA damage on rat hepatocytes following intravenous (iv) administration by using two in vivo genotoxicity assays: the unscheduled DNA synthesis (UDS) and the comet assays. The UDS assay measures DNA synthesis induced from the excision repair of DNA damaged regions and the comet assay is a very sensitive technique for detecting various forms of DNA damage. After an exposure time of 2-4 h or 12-16 h and a dose ranging from 12.5 to 50 microg/kg bw, no DNA damage could be observed in both assays on rat hepatocytes following iv administration. These findings have been discussed and compared with recently published genotoxic results obtained in other organs from mice after oral and intraperitoneal treatments to better understand the mechanism of action of this toxin in relation with its cancerogenicity potential.
Keywords : Comet assay Genotoxicity Microcystin-LR UDS assay
Type de document :
Article dans une revue
Environmental Toxicology, Wiley, 2009, 24 (2), pp.200-9. 〈10.1002/tox.20417〉
Liste complète des métadonnées
https://hal-anses.archives-ouvertes.fr/hal-00405465
Contributeur : Emmanuelle Chiffoleau <>
Soumis le : lundi 20 juillet 2009 - 15:14:46
Dernière modification le : lundi 20 juillet 2009 - 15:14:46

Lien texte intégral

istex

Identifiants

Collections

ANSES

Citation

Julien Gaudin, Ludovic Le Hegarat, Fabrice Nesslany, Daniel Marzin, Valérie Fessard. In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration.. Environmental Toxicology, Wiley, 2009, 24 (2), pp.200-9. 〈10.1002/tox.20417〉. 〈hal-00405465〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

102

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD