Validation of a multi-residue liquid chromatography-tandem mass spectrometry confirmatory method for 10 anticoccidials in eggs according to Commission Decision 2002/657/EC

Estelle Dubreil-Cheneau (1) , Mélaine Bessiral (1) , Brigitte Roudaut (1) , Eric Verdon (1) , Pascal Sanders (1)

1 Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants

A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the simultaneous detection and confirmation of halofuginone, robenidine, diclazuril, nicarbazin, monensin, narasin, lasalocid, salinomycin, maduramicin and semduramicin in whole egg has been developed and validated. The anticoccidial residues were extracted by acetonitrile, evaporated and dissolved in a sodium acetate/acetonitrile mixture. Then, the samples were injected on a C8 column in a gradient mode. Diclazuril-bis, DNC-d8 and nigericin were used as internal standards. The results of the full validation in accordance with the guidelines of the Commission Decision no 2002/657/EC are presented. This rapid and sensitive method was found suitable to confirm the anticoccidials at 1 and at 75mugkg(-1) for the MRL compound lasalocid.

Keywords: MULTI-RESIDUE ANALYSIS; liquid chromatography tandem mass spectrometry; analysis; residues; eggs; food; anticoccidial; policies; europe; confirmatory method

Domains: Analytical chemistry

Estelle Dubreil-Cheneau, Mélaine Bessiral, Brigitte Roudaut, Eric Verdon, Pascal Sanders. Validation of a multi-residue liquid chromatography-tandem mass spectrometry confirmatory method for 10 anticoccidials in eggs according to Commission Decision 2002/657/EC. Journal of Chromatography A, 2009, [Epub ahead of print]. ⟨hal-00405421⟩