Impact of therapeutic treatment with {beta}-lactam on transfer of the blaCTX-M-9 resistance gene from Salmonella enterica Virchow to Escherichia coli in gnotobiotic rats.

Stéphanie Faure 1 Agnès Perrin-Guyomard 1 Jean-Michel Delmas 1 Michel Laurentie 1
1 Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants
Abstract : Conjugative transfer of the plasmid carrying the blaCTX-M-9 gene from Salmonella enterica Virchow isolated from a chicken farm to a recipient Escherichia coli strain, was evaluated in vitro and in axenic rats inoculated with both strains, with and without selective pressure due to therapeutic doses of cefixime. The transfer of the blaCTX-M-9 gene of S. enterica Virchow to E. coli was confirmed in vitro, at a low frequency of 5.9 x 10(-8) transconjugants/donors. This transfer rate was higher in gnotobiotic rats and reached approximately 10(-5) transconjugants/donors without selective pressure. This frequency was unaffected by the addition of therapeutic doses of cefixime. Thus, estimates of in vitro transfer underestimated potential transfer in the digestive tract, and therapeutic doses of cefixime did not increase the selection for transconjugants.
keyword : antibioresistance antibiotic resistance salmonella enterica virchow escherichia coli gnotobiotic rat beta-lactan transfert transmission gene genetic
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Applied and Environmental Microbiology, American Society for Microbiology, 2009, epub ahead of print. 〈10.1128/AEM.00020-09〉
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Contributeur : Emmanuelle Chiffoleau <>
Soumis le : lundi 20 juillet 2009 - 14:25:38
Dernière modification le : lundi 20 juillet 2009 - 14:25:38

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Stéphanie Faure, Agnès Perrin-Guyomard, Jean-Michel Delmas, Michel Laurentie. Impact of therapeutic treatment with {beta}-lactam on transfer of the blaCTX-M-9 resistance gene from Salmonella enterica Virchow to Escherichia coli in gnotobiotic rats.. Applied and Environmental Microbiology, American Society for Microbiology, 2009, epub ahead of print. 〈10.1128/AEM.00020-09〉. 〈hal-00405401〉

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