Rapid typing of transmissible spongiform encephalopathy strains with differential ELISA.

Stéphanie Simon 1 Jérôme Nugier 1 Nathalie Morel 1 Hervé Boutal 1 Christophe Créminon 1 Sylvie L Benestad 2 Olivier Andréoletti 3, 4 Frédéric Lantier 5 Jean-Marc Bilheude 6 Muriel Feyssaguet 6 Anne-Gaëlle Biacabe 7 Thierry Baron 7, * Jacques Grassi 1
* Auteur correspondant
1 Commissariat à l'Energie Atomique
2 National Veterinary Institute
3 INRA - Institut National de la Recherche Agronomique
4 Ecole Nationale Vétérinaire de Toulouse
5 Infection Animale et Santé Publique
6 Bio-Rad
7 Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes
Abstract : The bovine spongiform encephalopathy (BSE) agent has been transmitted to humans, leading to variant Creutzfeldt-Jakob disease. Sheep and goats can be experimentally infected by BSE and have been potentially exposed to natural BSE; however, whether BSE can be transmitted to small ruminants is not known. Based on the particular biochemical properties of the abnormal prion protein (PrPsc) associated with BSE, and particularly the increased degradation induced by proteinase K in the N terminal part of PrPsc, we have developed a rapid ELISA designed to distinguish BSE from other scrapie strains. This assay clearly discriminates experimental ovine BSE from other scrapie strains and was used to screen 260 transmissible spongiform encephalopathy (TSE)-infected small ruminant samples identified by the French active surveillance network (2002/2003). In this context, this test has helped to identify the first case of natural BSE in a goat and can be used to classify TSE isolates based on the proteinase K sensitivity of PrPsc.
keyword : XUATNC HAFSSA
Type de document :
Article dans une revue
Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2008, 14 (4), pp.608-16
Liste complète des métadonnées

Littérature citée [36 références]  Voir  Masquer  Télécharger
https://hal-anses.archives-ouvertes.fr/hal-00373563
Contributeur : Valérie Cespedes <>
Soumis le : mardi 7 avril 2009 - 09:19:18
Dernière modification le : mercredi 24 mai 2017 - 01:08:04
Document(s) archivé(s) le : jeudi 10 juin 2010 - 19:51:47

Fichier

LPB888.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

  • HAL Id : hal-00373563, version 1
  • PUBMED : 18394279

Collections

INRA | ANSES | CEA

Citation

Stéphanie Simon, Jérôme Nugier, Nathalie Morel, Hervé Boutal, Christophe Créminon, et al.. Rapid typing of transmissible spongiform encephalopathy strains with differential ELISA.. Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2008, 14 (4), pp.608-16. 〈hal-00373563〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

432

Téléchargements de fichiers

129

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD