Utility of an internal control for evaluation of a Mycoplasma meleagridis PCR test. - Archive ouverte HAL Access content directly
Journal Articles Veterinary Microbiology Year : 1998

Utility of an internal control for evaluation of a Mycoplasma meleagridis PCR test.

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1

Abstract

Mycoplasma meleagridis, a turkey pathogen, can be detected by PCR directly from tracheal or genital swabs. However, up to 40% samples may contain inhibitory substances. A DNA fragment, that can be amplified with M. meleagridis primers and in the same cycling conditions, was constructed to use as an internal control (IC) to check for these inhibitors. This IC can easily be distinguished from the M. meleagridis amplicon after agarose gel electrophoresis since it is longer. Use of this IC in PCR amplifications revealed that more than 35% of turkey tracheal swabs and more than 45% of turkey cloacal swabs contained inhibitors. In most cases, dilution (1:100) of swab lysates allowed amplification of the internal control but DNA purification may be necessary to eliminate inhibitors (20% of tracheal swabs and 5% of cloacal swabs). Use of this internal control DNA allowed assessment of the efficiency of each individual reaction and ensured that the reaction was not inhibited by interfering substances.
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Dates and versions

hal-00366206 , version 1 (06-03-2009)

Identifiers

  • HAL Id : hal-00366206 , version 1
  • PUBMED : 9646464

Cite

P. Y. Moalic, F. Gesbert, I. Kempf. Utility of an internal control for evaluation of a Mycoplasma meleagridis PCR test.. Veterinary Microbiology, 1998, 61 (1-2), pp.41-9. ⟨hal-00366206⟩

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