Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment

Anne-Laure Boutigny (1) , Benoît Remenant (1) , Bruno Legendre (1) , Véronique Béven (2) , Mathieu Rolland (1) , Yannick Blanchard (2) , Amandine Cunty (1)

1 BVO - Unité de bactériologie, virologie, OGM
2 Laboratoire de Ploufragan-Plouzané-Niort [ANSES]

A targeted enrichment method was developed to sequence Xylella fastidiosa genomic DNA directly from plant samples. The method was evaluated on various plant species infected with different strains at different levels of contamination. After enrichment, X. fastidiosa genome coverage was above 99.9% for all tested samples.

Mots clés: Capture; Illumina sequencing; SureSelect; Whole genome; Xylella fastidiosa

Domaines: Sciences du Vivant [q-bio]

Anne-Laure Boutigny, Benoît Remenant, Bruno Legendre, Véronique Béven, Mathieu Rolland, et al.. Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment. Journal of Microbiological Methods, 2023, 208, pp.106719. ⟨10.1016/j.mimet.2023.106719⟩. ⟨anses-04074627⟩