Draft genome of a ST443 mcr-1 - and bla CTX-M-2 -carrying Escherichia coli from cattle in Brazil

Josman Dantas Palmeira (1) , Helena Ferreira (1) , Jean-Yves Madec (2) , Marisa Haenni (2)

1 Universidade do Porto = University of Porto
2 AVB - Unité Antibiorésistance et Virulence Bactériennes

Objectives: Colistin is used in Brazil for the treatment of food-producing animals. The colistin resistance gene mcr-1 has already been reported from chicken and swine in this country. Here we report the draft genome of an Escherichia coli isolate presenting both an extended-spectrum β-lactamase (ESBL) gene and the mcr-1 gene in a healthy cow in Brazil. Methods: Whole genomic DNA from E. coli E12 was extracted and 2Â 150-bp paired-end reads were generated using Illumina sequencing technology. De novo genome assembly was performed using SPAdes v.3.11 and the draft genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Further analyses were performed using Center for Genomic Epidemiology databases. Southern blots were performed to characterise plasmid location. Results: The 5 024 393-bp genome displayed several resistance genes, including the mcr-1 and bla CTX-M-2 genes. These two genes were located on different plasmids (mcr-1 on an IncX4 plasmid and bla CTX-M-2 on an IncF plasmid). Conclusion: The genome sequence reported here can be compared with previously published genomes for mcr-1-producing isolates. This will ultimately help to understand the routes of dissemination of the resistance genes.

Keywords: mcr-1; Colistin; CTX-M-2; ESBL; Cow; Brazil

Domains: Bacteriology

Josman Dantas Palmeira, Helena Ferreira, Jean-Yves Madec, Marisa Haenni. Draft genome of a ST443 mcr-1 - and bla CTX-M-2 -carrying Escherichia coli from cattle in Brazil. Journal of Global Antimicrobial Resistance, 2018, 13, pp.269 - 270. ⟨10.1016/j.jgar.2018.05.010⟩. ⟨anses-04013204⟩