Pandemic Escherichia coli ST648 isolate harbouring fosA3 and blaCTX-M-8 on an IncI1/ST113 plasmid: A new successful combination for the spread of fosfomycin resistance?

Josman Dantas Palmeira (1) , Helena Ferreira (1) , Jean-Yves Madec (2, 3) , Marisa Haenni (2, 3)

1 Universidade do Porto = University of Porto
2 Université de Lyon
3 AVB - Unité Antibiorésistance et Virulence Bactériennes

Objectives: The emergence of Enterobacteriaceae isolates resistant to the last-resort antibiotic fosfomycin outside of Asia is a public-health issue. Here we report the draft genome of an Escherichia coli isolate presenting both an extended-spectrum β-lactamase (ESBL) and the fosA3 gene in a healthy cow in Brazil. Methods: Whole genomic DNA from E. coli E12 was extracted and 2 Â 150-bp paired-end reads were generated using Illumina sequencing technology. De novo genome assembly was performed using SPAdes v.3.11 and the draft genome was annotated by the NCBI Prokaryotic Genome Annotation Pipeline. Further analyses were performed using the Center for Genomic Epidemiology databases. Results: The 5 045 934-bp genome displayed several resistance genes, including the fosA3 and bla CTX-M-8 genes. Southern blot experiments showed that they were co-located on an IncI1/ST113 plasmid. Conclusion: Presence of the fosA3 gene on the same common plasmid as bla CTX-M-8 will have to be monitored. This draft genome provides data that will help in tracing the dissemination of this gene and the evolution of its plasmidic support.

Keywords: fosA3; Fosfomycin; CTX-M-8; ESBL; Cow; Brazil

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Josman Dantas Palmeira, Helena Ferreira, Jean-Yves Madec, Marisa Haenni. Pandemic Escherichia coli ST648 isolate harbouring fosA3 and blaCTX-M-8 on an IncI1/ST113 plasmid: A new successful combination for the spread of fosfomycin resistance?. Journal of Global Antimicrobial Resistance, 2018, 15, pp.254 - 255. ⟨10.1016/j.jgar.2018.10.025⟩. ⟨anses-03993321⟩