Co-Occurrence of Francisella, Spotted Fever Group Rickettsia, and Midichloria in Avian-Associated Hyalomma rufipes - Archive ouverte HAL Access content directly
Journal Articles Microorganisms Year : 2022

Co-Occurrence of Francisella, Spotted Fever Group Rickettsia, and Midichloria in Avian-Associated Hyalomma rufipes

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Tove Hoffman
Andreas Sjödin
Caroline Öhrman
Linda Karlsson
  • Function : Author
Ryelan Francis Mcdonough
  • Function : Author
Jason Sahl
  • Function : Author
Dawn Birdsell
  • Function : Author
David Wagner
Laura Carra
  • Function : Author
Peter Wilhelmsson
  • Function : Author
John H.-O. Pettersson
  • Function : Author
Christos Barboutis
Jordi Figuerola
Alejandro Onrubia
Yosef Kiat
Dario Piacentini
  • Function : Author
Thomas Jaenson
  • Function : Author
Per-Eric Lindgren
Thord Fransson
Mats Forsman
Kenneth Nilsson
  • Function : Author
Åke Lundkvist
Björn Olsen
  • Function : Author

Abstract

The migratory behavior of wild birds contributes to the geographical spread of ticks and their microorganisms. In this study, we aimed to investigate the dispersal and co-occurrence of Francisella and spotted fever group Rickettsia (SFGR) in ticks infesting birds migrating northward in the African-Western Palaearctic region (AWPR). Birds were trapped with mist nests across the Mediterranean basin during the 2014 and 2015 spring migration. In total, 575 ticks were collected from 244 birds. We screened the ticks for the species Francisella tularensis, the genus Francisella, and SFGR by microfluidic real-time PCR. Confirmatory analyses and metagenomic sequencing were performed on tick samples that putatively tested positive for F. tularensis during initial screenings. Hyalomma rufipes was the most common tick species and had a high prevalence of Francisella, including co-occurrence of Francisella and SFGR. Metagenomic analysis of total DNA extracted from two H. rufipes confirmed the presence of Francisella, Rickettsia, and Midichloria. Average nucleotide identity and phylogenetic inference indicated the highest identity of the metagenome-assembled genomes to a Francisella-like endosymbiont (FLE), Rickettsia aeschlimannii, and Midichloria mitochondrii. The results of this study suggest that (i) FLE- and SFGR-containing ticks are dispersed by northbound migratory birds in the AWPR, (ii) H. rufipes likely is not involved in transmission of F. tularensis in the AWPR, and (iii) a dual endosymbiosis of FLEs and Midichloria may support some of the nutritional requirements of H. rufipes.

Dates and versions

anses-03942987 , version 1 (17-01-2023)

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Tove Hoffman, Andreas Sjödin, Caroline Öhrman, Linda Karlsson, Ryelan Francis Mcdonough, et al.. Co-Occurrence of Francisella, Spotted Fever Group Rickettsia, and Midichloria in Avian-Associated Hyalomma rufipes. Microorganisms, 2022, 10 (7), pp.1393. ⟨10.3390/microorganisms10071393⟩. ⟨anses-03942987⟩

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