Detection of West Nile Virus Lineage 2 in Eastern Romania and First Identification of Sindbis Virus RNA in Mosquitoes Analyzed using High-Throughput Microfluidic Real-Time PCR - Archive ouverte HAL Access content directly
Journal Articles Viruses Year : 2023

Detection of West Nile Virus Lineage 2 in Eastern Romania and First Identification of Sindbis Virus RNA in Mosquitoes Analyzed using High-Throughput Microfluidic Real-Time PCR

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1
Luciana Alexandra Crivei
  • Function : Author
Gaëlle Gonzalez
  • Function : Author
Steeve Lowenski
  • Function : Author
Ioana Cristina Crivei
  • Function : Author
Daniela Porea
  • Function : Author
Dragoș Constantin Anita
Ioana Alexandra Ratoi
  • Function : Author
Stéphan Zientara
Luanda Elena Oslobanu
  • Function : Author
Alexandru Tomazatos
  • Function : Author
Gheorghe Savuta
  • Function : Author
Sylvie Lecollinet

Abstract

The impact of mosquito-borne diseases on human and veterinary health is being exacerbated by rapid environmental changes caused mainly by changing climatic patterns and globalization. To gain insight into mosquito-borne virus circulation from two counties in eastern and southeastern Romania, we have used a combination of sampling methods in natural, urban and peri-urban sites. The presence of 37 mosquito-borne viruses in 16,827 pooled mosquitoes was analyzed using a high-throughput microfluidic real-time PCR assay. West Nile virus (WNV) was detected in 10/365 pools of Culex pipiens (n = 8), Culex modestus (n = 1) and Aedes vexans (n = 1) from both studied counties. We also report the first molecular detection of Sindbis virus (SINV) RNA in the country in one pool of Culex modestus. WNV infection was confirmed by real-time RT-PCR (10/10) and virus isolation on Vero or C6/36 cells (four samples). For the SINV-positive pool, no cytopathic effectwas observed after infection of Vero or C6/36 cells, but no amplification was obtained in conventional SINV RT-PCR. Phylogenetic analysis of WNV partial NS5 sequences revealed that WNV lineage 2 of theCentral-Southeast European clade, has a wider circulation in Romania than previously known.

Dates and versions

anses-03942949 , version 1 (17-01-2023)

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Luciana Alexandra Crivei, Sara Moutailler, Gaëlle Gonzalez, Steeve Lowenski, Ioana Cristina Crivei, et al.. Detection of West Nile Virus Lineage 2 in Eastern Romania and First Identification of Sindbis Virus RNA in Mosquitoes Analyzed using High-Throughput Microfluidic Real-Time PCR. Viruses, 2023, 15 (1), pp.186. ⟨10.3390/v15010186⟩. ⟨anses-03942949⟩

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