Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model - Archive ouverte HAL Access content directly
Journal Articles Environmental Toxicology and Pharmacology Year : 2022

Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model

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Abstract

Human intoxications in the Mediterranean Sea have been linked to blooms of the dinoflagellate Ostreopsis cf. ovata, producer of palytoxin (PlTX)-like toxins called ovatoxins (OVTXs). Exposure routes include only inhalation and contact, although PlTX-poisoning by seafood has been described in tropical regions. To address the impact of OVTXs on the intestinal barrier, dinoflagellate extracts, purified OVTX-a and -d and PlTX were tested on differentiated Caco-2 cells. Viability, inflammatory response and barrier integrity were recorded after 24h treatment. OVTX-a and -d were not cytotoxic up to 20ng/mL but increased IL-8 release, although to a lesser extent compared to PlTX. While PlTX and OVTX-a (at 0.5 and 5ng/mL respectively) affected intestinal barrier integrity OVTX-d up to 5ng/mL did not. Overall, OVTX-d was shown to be less toxic than OVTX-a and PlTX. Therefore, oral exposure to OVTX-a and -d could provoked lower acute toxicity than PlTX.
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anses-03701528 , version 1 (22-06-2022)

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Marin-Pierre Gemin, Rachelle Lanceleur, Lisa Meslier, Fabienne Herve, Damien Reveillon, et al.. Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model. Environmental Toxicology and Pharmacology, 2022, 94, pp.103909. ⟨10.1016/j.etap.2022.103909⟩. ⟨anses-03701528⟩
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