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A Trichinella spiralis new born larvae-specific protein, Ts-NBL1, interacts with host’s cell vimentin

Abstract : Abstract The parasitic nematode Trichinella has a special relationship with its host as it has a unique intracellular location within the feeder cell which is a structure derived from skeletal muscle fiber. It has been proposed that “parakines” secreted by Trichinella larvae serve as messengers to implement communication between the parasite and the muscle cells through a molecular cross-talk to ensure permanent coexistence within the host. The Ts- NBL1 protein is considered to be a potential key “parakine” involved in the early invasion of the muscle fiber and its transformation into a feeder cell during Trichinella spiralis infection. This study used for the first time yeast two-hybrid (Y2H) technology in Trichinella to identify Ts- NBL1 interacting proteins. GST co-affinity purification experiments confirmed vimentin as an important interactor. The discovery of the new host proteins interacting with Ts- NBL1 will help to suggest that Ts- NBL1 contributes to participate in the capsule formation of feeder cells and provide ideas for understanding the molecular and cellular mechanisms involved in the survival of Trichinella in the host.
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https://hal-anses.archives-ouvertes.fr/anses-03659181
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Submitted on : Wednesday, May 4, 2022 - 4:16:20 PM
Last modification on : Friday, August 19, 2022 - 1:40:05 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Anqi Wang, Xiaolei Liu, Aurélie Heckmann, Grégory Caignard, Damien Vitour, et al.. A Trichinella spiralis new born larvae-specific protein, Ts-NBL1, interacts with host’s cell vimentin. Parasitology Research, Springer Verlag (Germany), 2022, 121 (5), pp.1369-1378. ⟨10.1007/s00436-022-07479-7⟩. ⟨anses-03659181⟩

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