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Method for tick-borne encephalitis virus detection in raw milk products

Abstract : The transmission of tick-borne encephalitis virus (TBEV) through food is rare, but can occur through the consumption of raw milk products from animals infected by tick bites. In 2020, France faced a TBEV outbreak linked to the consumption of unpasteurized goat cheese. The aim of this study was to develop and characterize a molecular method for the detection of TBEV in raw milk products based on the recent international standard PR ISO/DIS 16140-4. The TBEV recovery rates varied with the inoculation level and settings. The LOD50 and LOD95 of TBEV were 6.40 × 103 genome copies per g or per mL and 2.84 × 104 genome copies per g or per mL, respectively. The percentages of RT-qPCR inhibitions were lower than 75% and the murine norovirus (MNV-1), used as process control, was detected in all samples with a recovery rate higher than 1%, as recommended in ISO 15216. We conclude that the described method is appropriate to detect TBEV in raw milk products for routine diagnosis, and to assess potential health risks.
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https://hal-anses.archives-ouvertes.fr/anses-03648882
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Submitted on : Friday, April 22, 2022 - 9:35:11 AM
Last modification on : Saturday, April 23, 2022 - 3:30:29 AM

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Catherine Hennechart-Collette, Gaëlle Gonzalez, Lisa Fourniol, Audrey Fraisse, Cécile Beck, et al.. Method for tick-borne encephalitis virus detection in raw milk products. Food Microbiology, Elsevier, 2022, 104, pp.104003. ⟨10.1016/j.fm.2022.104003⟩. ⟨anses-03648882⟩

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