MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples

Thomas Brauge (1, 2) , Sylvain Trigueros (1, 2) , Arnaud Briet (1, 2) , Sabine Debuiche (1, 2) , Guylaine Leleu (1, 2) , Benoit Gassilloud (3) , Amandine Wilhelm (3) , Jean-Sébastien Py (3) , Graziella Midelet (1, 2)

1 LSAl - Laboratoire de sécurité des aliments de Maisons-Alfort
2 Laboratoire de Boulogne sur mer
3 Laboratoire d'hydrologie de Nancy

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) associated with the Bruker BioTyper TM V7.0.0 database for the identification of 713 bacterial strains isolated from seafood products and sea water samples (ANSES B3PA collection) under culture conditions that may have been significantly different from those used to create the reference spectrum vs. the 16S rDNA sequencing. We identified 78.8% of seafood isolates with 46.7% at the species level (Bruker score above 2) and 21.2% (Bruker score between 1.7 and 2) at the genus level by the two identification methods, except for 3.8% of isolates with a difference of identification between the two methods (Bruker score between 1.7 and 2). There were 41.9% isolates (Bruker score below 1.7) with the identification at the genus level. We identified 94.4% of seafood isolates with 16S rDNA sequencing. The MALDI-TOF allowed a better strain identification to the species level contrary to the 16s rDNA sequencing, which allowed an identification mainly to the genus level. MALDI-TOF MS in association with the Bruker database and 16S rDNA sequencing are powerful tools to identify a wide variety of bacteria from seafood but require further identification by biochemical, molecular technique or other conventional tests.

Keywords: marine microbiology; seafood; sea water; ecology; bacterial identification; 16s rDNA sequencing; food pathogens and spoilage; MALDI-TOF MS

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Thomas Brauge, Sylvain Trigueros, Arnaud Briet, Sabine Debuiche, Guylaine Leleu, et al.. MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples. Frontiers in Marine Science, 2021, 8, ⟨10.3389/fmars.2021.650116⟩. ⟨anses-03524181⟩