The Use of Flocked Swabs with a Protective Medium Increases the Recovery of Live Brucella spp. and DNA Detection

Luca Freddi (1, 2) , Vitomir Djokic (1, 2) , Fathia Petot-Bottin (1, 2) , Guillaume Girault (1, 2) , Ludivine Perrot (1, 2) , Acacia Ferreira Vicente (1, 2) , Claire Ponsart (1, 2)

1 Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie
2 UPE - Université Paris-Est

In order to protect public and veterinary health from highly zoonotic bacteria such as members of the genus Brucella and prevent their dissemination into the environment, direct diagnostics are of utmost importance. However, in addition to the highly specific diagnostic tests, the sampling methods, time necessary for specimens to reach the laboratories, and transport conditions are important factors to consider in order to increase the sensitivity of performed tests, especially bacterial culturing and qPCR.

Keywords: swab; Brucella; storage condition; recovery; qPCR; bacteriology

Domains: Life Sciences [q-bio]; Animal biology; Veterinary medicine and animal Health

Luca Freddi, Vitomir Djokic, Fathia Petot-Bottin, Guillaume Girault, Ludivine Perrot, et al.. The Use of Flocked Swabs with a Protective Medium Increases the Recovery of Live Brucella spp. and DNA Detection. Microbiology Spectrum, 2021, pp.e0072821. ⟨10.1128/Spectrum.00728-21⟩. ⟨anses-03451300⟩