Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice - Archive ouverte HAL Access content directly
Journal Articles Journal of Veterinary Medical Science Year : 2018

Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice

(1) , (1) , (1) , (2, 1) , (2) , (2, 1) , (1) , (1) , (3) , (2)
1
2
3

Abstract

In 2013, the first case of Taiwan ferret badger rabies virus (RABV-TWFB) infection was reported in Formosan ferret badgers, and two genetic groups of the virus were distinguished through phylogenetic analysis. To detect RABV-TWFB using a sensitive nucleic acid-based method, a quantitative real-time reverse transcription polymerase chain reaction targeting the conserved region of both genetic groups of RABV-TWFB was developed. This method had a limit of detection (LOD) of 40 RNA copies/reaction and detected viral RNA in brain and ear tissue specimens of infected and dead Formosan ferret badgers and mice with 100% sensitivity and specificity. The mean viral RNA load detected in the ear tissue specimens of ferret badgers ranged from 3.89 × 108 to 9.73 × 108 RNA copies/g-organ, which was 111-fold to 2,220-fold lower than the concentration detected in the brain specimens, but 2,000-fold to 5,000-fold higher than the LOD of the assay. This highly sensitive technique does not require facilities or instruments complying with strict biosafety criteria. Furthermore, it is efficient, safe, and labor-saving as only ear specimens need be sampled. Therefore, it is a promising technique for epidemiological screening of Taiwan ferret badger rabies.
Fichier principal
Vignette du fichier
80_17-0539.pdf (1.36 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

anses-03409074 , version 1 (29-10-2021)

Identifiers

Cite

Ai-Ping Hsu, Chun-Hsien Tseng, Yi-Ta Lu, Yu-Hua Shih, Chung-Hsi Chou, et al.. Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice. Journal of Veterinary Medical Science, 2018, 80 (6), pp.1012-1019. ⟨10.1292/jvms.17-0539⟩. ⟨anses-03409074⟩

Collections

ANSES
15 View
22 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More