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Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice

Abstract : In 2013, the first case of Taiwan ferret badger rabies virus (RABV-TWFB) infection was reported in Formosan ferret badgers, and two genetic groups of the virus were distinguished through phylogenetic analysis. To detect RABV-TWFB using a sensitive nucleic acid-based method, a quantitative real-time reverse transcription polymerase chain reaction targeting the conserved region of both genetic groups of RABV-TWFB was developed. This method had a limit of detection (LOD) of 40 RNA copies/reaction and detected viral RNA in brain and ear tissue specimens of infected and dead Formosan ferret badgers and mice with 100% sensitivity and specificity. The mean viral RNA load detected in the ear tissue specimens of ferret badgers ranged from 3.89 × 108 to 9.73 × 108 RNA copies/g-organ, which was 111-fold to 2,220-fold lower than the concentration detected in the brain specimens, but 2,000-fold to 5,000-fold higher than the LOD of the assay. This highly sensitive technique does not require facilities or instruments complying with strict biosafety criteria. Furthermore, it is efficient, safe, and labor-saving as only ear specimens need be sampled. Therefore, it is a promising technique for epidemiological screening of Taiwan ferret badger rabies.
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https://hal-anses.archives-ouvertes.fr/anses-03409074
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Submitted on : Friday, October 29, 2021 - 2:37:37 PM
Last modification on : Wednesday, November 3, 2021 - 4:01:19 AM

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Ai-Ping Hsu, Chun-Hsien Tseng, Yi-Ta Lu, Yu-Hua Shih, Chung-Hsi Chou, et al.. Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice. Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 2018, 80 (6), pp.1012-1019. ⟨10.1292/jvms.17-0539⟩. ⟨anses-03409074⟩

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