Detection of rabies antibodies in wild boars in north-east Romania by a rabies ELISA test - Anses - Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail Access content directly
Journal Articles BMC Veterinary Research Year : 2019

Detection of rabies antibodies in wild boars in north-east Romania by a rabies ELISA test


Abstract Background In the last few decades, Romania has been considered one of the European countries most affected by animal rabies, but a combination of oral rabies vaccination (ORV) campaigns in foxes alongside mandatory vaccination of pets has substantially decreased the number of rabies cases in recent years. The objective of this study was to detect rabies antibodies in wild boar serum and thoracic fluid samples collected during the hunting season after ORV campaigns in north-eastern Romania in order to identify if wild boars are substantial competitors to foxes for ORV baits. Results When the 312 wild boar samples were tested by ELISA (BioPro ELISA, Czech Republic), 42.31% (132/312) demonstrated rabies antibodies. In order to compare these wild boar results in terms of the percentage of immunisation, fox samples were also included in the study, and in this case only 28.40% (98/345) demonstrated rabies antibodies by ELISA. To check the diagnostic sensitivity and specificity of this ELISA, those samples with a sufficient volume from both species that had tested either negative or positive with an initial ELISA were then tested with the Fluorescent Antibody Virus Neutralisation (FAVN) assay. The overall concordance between the BioPro ELISA and FAVN test was 74.26% (75/101) in wild boar samples and 65.66% (65/99) in fox samples, 140 out of 200 samples being correlated with the two methods, although no significant statistical difference ( p = 0.218) between the two species was registered. We found a good agreement by both tests for the ELISA-positive samples (91.30%), however the situation was different for the ELISA-negative samples, where a low agreement was demonstrated (41.18%). Conclusions This study reports for the first time the presence of rabies antibodies in wild boar samples collected during the hunting season in Romania after ORV campaigns in rabies endemic areas. It is also the first study to demonstrate that ELISA BioPro can be used on wild boar samples with satisfactory results compared to the FAVN test for this species.
Fichier principal
Vignette du fichier
s12917-019-2209-x.pdf (881.27 Ko) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

anses-03297275 , version 1 (20-10-2021)



Mihaela Anca Dascalu, Marine Wasniewski, Evelyne Picard-Meyer, Alexandre Servat, Florentina Daraban Bocaneti, et al.. Detection of rabies antibodies in wild boars in north-east Romania by a rabies ELISA test. BMC Veterinary Research, 2019, 15 (1), pp.466. ⟨10.1186/s12917-019-2209-x⟩. ⟨anses-03297275⟩


24 View
60 Download



Gmail Facebook Twitter LinkedIn More