Skip to Main content Skip to Navigation
Journal articles

Genomic elements located in the accessory repertoire drive the adaptation to biocides in Listeria monocytogenes strains from different ecological niches

Abstract : In response to the massive use of biocides for controlling Listeria monocytogenes (hereafter Lm) contaminations along the food chain, strains showing biocide tolerance emerged. Here, accessory genomic elements were associated with biocide tolerance through pangenome-wide associations performed on 197 Lm strains from different lineages, ecological, geographical and temporal origins. Mobile elements, including prophage-related loci, the Tn6188_qacH transposon and pLMST6_emrC plasmid, were widespread across lineage I and II food strains and associated with tolerance to benzalkonium-chloride (BC), a quaternary ammonium compound (QAC) widely used in food processing. The pLMST6_emrC was also associated with tolerance to another QAC, the didecyldimethylammonium-chloride, displaying a pleiotropic effect. While no associations were detected for chemically reactive biocides (alcohols and chlorines), genes encoding for cell-surface proteins were associated with BC or polymeric biguanide tolerance. The latter was restricted to lineage I strains from animal and the environment. In conclusion, different genetic markers, with polygenic nature or not, appear to have driven the Lm adaptation to biocide, especially in food strains but also from animal and the environment. These markers could aid to monitor and predict the spread of biocide tolerant Lm genotypes across different ecological niches, finally reducing the risk of such strains in food industrial settings.
Document type :
Journal articles
Complete list of metadata

https://hal-anses.archives-ouvertes.fr/anses-03150893
Contributor : Sophie Guitton <>
Submitted on : Wednesday, February 24, 2021 - 11:17:43 AM
Last modification on : Thursday, February 25, 2021 - 3:28:04 AM

Links full text

Identifiers

Collections

Citation

Federica Palma, Nicolas Radomski, Alizée Guérin, Yann Sévellec, Benjamin Félix, et al.. Genomic elements located in the accessory repertoire drive the adaptation to biocides in Listeria monocytogenes strains from different ecological niches. Food Microbiology, Elsevier, 2021, pp.103757. ⟨10.1016/j.fm.2021.103757⟩. ⟨anses-03150893⟩

Share

Metrics

Record views

20