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Article Dans Une Revue Microbial ecology Année : 2020

Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms

Résumé

Genetic diversification through the emergence of variants is one of the known mechanisms enabling the adaptation of bacterial communities. We focused in this work on the adaptation of the model strainPseudomonas putidaKT2440 in association with anotherP. putidastrain (PCL1480) recently isolated from soil to investigate the potential role of bacterial interactions in the diversification process. On the basis of colony morphology, three variants ofP. putidaKT2440 were obtained from co-culture after 168 h of growth whereas no variant was identified from the axenic KT2440 biofilm. The variants exhibited distinct phenotypes and produced biofilms with specific architecture in comparison with the ancestor. The variants better competed with theP. putidaPCL1480 strain in the dual-strain biofilms after 24 h of co-culture in comparison with the ancestor. Moreover, the synergistic interaction of KT2440 ancestor and the variants led to an improved biofilm production and to higher competitive ability versus the PCL1480 strain, highlighting the key role of diversification in the adaptation ofP. putidaKT2440 in the mixed community. Whole genome sequencing revealed mutations in polysaccharides biosynthesis protein, membrane transporter, or lipoprotein signal peptidase genes in variants.
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hal-03031204 , version 1 (30-11-2020)

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Arnaud Bridier, J. Piard, Romain Briandet, T. Bouchez. Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms. Microbial ecology, 2020, 80 (1), pp.47-59. ⟨10.1007/s00248-019-01470-z⟩. ⟨hal-03031204⟩
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