Phenotypic and genotypic characterization of H2S-positive and H2S-negative strains of Shewanella baltica isolated from spoiled whiting ( Merlangius merlangus )

Alexandre Dehaut (1) , Graziella Midelet-Bourdin (1) , Anne Brisabois (1) , Guillaume Duflos (1)

1 LSAl - Laboratoire de sécurité des aliments de Maisons-Alfort

Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species but showed that one of the four strains was unable to produce H2 S. Two strains synthetized an ornithine decarboxylase being potential putrescine producers. Results of carbon source use highlighted that the four strains were able to use citrate and d-sucrose and one strain was not able to use l-arabinose. Genotypic characterization of the strains thanks to 16S rRNA and gyrB partial gene sequencing led to their identification as members of Shewanella baltica species. These observations suggest that H2 S production may not be the most appropriate screening parameter for Shewanella species and further to monitor the development of spoilage flora.

Keywords: H2S-negative strain; 16S rRNA gene; Shewanella baltica; Taxonomy; Fish spoilage; gyrB gene

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Biochemistry [q-bio.BM]

Alexandre Dehaut, Graziella Midelet-Bourdin, Anne Brisabois, Guillaume Duflos. Phenotypic and genotypic characterization of H2S-positive and H2S-negative strains of Shewanella baltica isolated from spoiled whiting ( Merlangius merlangus ). Letters in Applied Microbiology, 2014, 59 (5), pp.542 - 548. ⟨10.1111/lam.12312⟩. ⟨anses-01889897⟩