Les oligodendrocytes sont-ils concernés par l'accumulation d'alpha-synucleine pathologique dans le cerveau de souris TGM83 : études immunohistochimiques comparées

Abstract : To study the possible impact of chemical substances, like pesticides or nanoparticules (NP), on human neurodegenerative diseases associated to abnormal expression of the alpha-synuclein protein (Parkinson’s disease, Lewy body dementia, multi-systematized atrophy), our laboratory uses experimental analogues. Among them the C57Bl/6 wild-type mouse line and the TgM83 transgenic mouse line that expresses a mutant A53T form of human alpha-synuclein are studied. Realistic expo-sures are performed using oral route. First histological analyses of brains of exposed TgM83 mice reveal the presence of gliosis, brain inflammation as well as accumulation of pathological form of alpha-synuclein. Beside classical neuronal expression of this abnormal alpha-synuclein in various gray matter areas, a labeling identified in the white matter points out that oligodendrocytes may be also concerned by the alpha-synuclein related disorder. This observation evokes the typical labeling reported in the brain of humans with multi-systematized atrophy. Thus to test the assumption of a possible implication of the oligodendrocytes in the pathological accumulation of alpha-synuclein in the TgM83 model, we set up a protocol of oligodendrocyte detection using immunohistochemistry. Here we report the optimal conditions determined for an application on fixed paraffin embedded tissue samples that was used consecutively in a strategy of multiple labeling. To deal with a major methodological difficulty resting on the use of primary antibodies produced in the same species, we tested the use of a dedicated commercial kit. We report here not only the principle and details of application of this kit, but also the advantages and the limits identified.
Document type :
Journal articles
Complete list of metadatas

Cited literature [14 references]  Display  Hide  Download

https://hal-anses.archives-ouvertes.fr/anses-01874783
Contributor : Anna Bencsik <>
Submitted on : Thursday, April 18, 2019 - 5:35:06 PM
Last modification on : Wednesday, May 8, 2019 - 1:24:17 AM

File

valantin29.pdf
Explicit agreement for this submission

Identifiers

  • HAL Id : anses-01874783, version 1

Collections

Citation

Julie Valantin, A. Bencsik, Mickael Leboidre. Les oligodendrocytes sont-ils concernés par l'accumulation d'alpha-synucleine pathologique dans le cerveau de souris TGM83 : études immunohistochimiques comparées. Revue Française d'Histotechnologie, Association française d'histotechnologie, 2015, 27 (1), pp.29-45. ⟨anses-01874783⟩

Share

Metrics

Record views

30

Files downloads

9