In vitro and in vivo genotoxicity of nano aluminum, aluminum oxide and aluminum chloride: A comparative study
Résumé
Volume 280, Supplement 1, Pages S1-S346 (20 October 2017)
Abstracts of the 53rd Congress of the European Societies of Toxicology (EUROTOX)Bratislava, Slovakia, 10th–13th September, 2017
Edited by Mumtaz Iscan and Helena Kandarova
P-03-02-16
Aluminum on different forms is found in several goods: food addi- tives, medication, beverage, water treatment as well as cooking utensils. EFSA has established a tolerable weekly intake (TWI) of 1 mg/kg bw for human oral exposure (EFSA, 2008). Since the 2000s, due to the increasing use of nanoparticles (NP), aluminum NPs are expected to be more largely involved in human exposure although their effects on human health has not been fully characterized. Nevertheless, aluminum was depicted to cross epithelial barriers and to include neurotoxicity and embryotoxic- ity. As aluminum NPs may not behave as Al ions, their toxic effects must be investigated. In this study, we investigated the genotoxicity of Al, Al 2 O 3 NPs and the ionic form AlCl 3 on intestine, the contact organ fol- lowing oral exposure, and on liver, the main target organ for Al accumulation using both the alkaline comet assay and the Fpg- modified comet assay for highlighting potential oxidative damage. We observed DNA damage on the human intestinal Caco2 and hepatic HepaRG cells (exposure from 0.6 to 256 g/cm 2 ). How- ever, no genotoxicity was detected in duodenum and liver of male Sprague Dawley rats after 3 gavages as well as after 28 days oral treatment with 6, 12 and 25 mg/kg bw/day. This project was funded by Agence Nationale de la Recherche (ANR-13-IS10-0005-01).