Reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds

Christophe Soumet 1 Delphine Méheust 2 Christine Pissavin 2 Patricia Legrandois 1 B. Fremaux 3 Carole Feurer 3 A. Le Roux 3 M Denis 4 Pierre Maris 1
1 ANSES - ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
2 Département Génie Biologique / IUT de Saint-Brieuc
3 Institut du Porc
4 Laboratoire de Ploufragan - Plouzané
Abstract : Aims: Our aim was to assess the effects of step-wise exposure to didecyl dimethyl ammonium chloride (DDAC) on the antimicrobial (antibiotics and biocides) susceptibilities of food-associated bacterial strains. Methods and Results: Adaptive responses of bacterial strains were investigated by exposing the strains daily to increasing subinhibitory concentrations of DDAC for 7 days. Following adaptation to DDAC, a threefold increase in the minimum inhibitory concentration (MIC) values for this biocide was observed in 48% of the Escherichia coli and Listeria monocytogenes strains, and 3% of the Salmonella strains. Reduced susceptibility to other biocides was found with the most important increase in MIC for benzalkonium chloride (BC) and a commercial biocide formulation (Galox Horizon) containing DDAC and glutaraldehyde, for all species except Salmonella. Increase in antibiotic MIC values was more pronounced in E. coli in terms of antibiotic numbers and of magnitude (from 4- to 32-fold increase) and, to a lesser extent, in Salmonella strains. Most of these strains had acquired resistance to ampicillin, cefotaxime, ceftazidime, chloramphenicol and ciprofloxacin. Conclusions: The effects of exposure to DDAC on biocides and antibiotics susceptibilities depend upon the bacteria species. Significance and Impact of the Study: Extensive use of DDAC at subinhibitory concentrations may lead to the development of antibiotic-resistant bacteria and may represent a public health issue.
Keywords : bacteria susceptibility antibiotic resistance food safety quaternary ammonium disinfectant
Document type :
Journal articles
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https://hal-anses.archives-ouvertes.fr/anses-01419300
Contributor : Emmanuelle Chiffoleau <>
Submitted on : Monday, December 19, 2016 - 11:23:04 AM
Last modification on : Tuesday, February 19, 2019 - 3:20:02 PM

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ANSES | UNIV-RENNES1 | UNIV-RENNES

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Christophe Soumet, Delphine Méheust, Christine Pissavin, Patricia Legrandois, B. Fremaux, et al.. Reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds. Journal of Applied Microbiology, Wiley, 2016, 121 (5), pp.1275-1281. ⟨10.1111/jam.13247⟩. ⟨anses-01419300⟩

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