In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions

K Kittler Dominique Hurtaud Pessel 1 R Maul F Kolrep Valérie Fessard, 1
1 ANSES - ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
Abstract : No evidence for phase I metabolites of the cyanotoxin cylindrospermopsin (CYN) was given using HepaRG cells and different liver tissue fractions when studying metabolic conversion. Although the application of ketoconazole, a CYP3A4 inhibitor, led to a decreased cytotoxicity of CYN, no metabolites were detected applying high resolution mass spectrometry. Quantification of non-modified CYN led to recovery rates of almost 100%. Consequently, reduction of CYN toxicity in the presence of metabolism inhibiting agents must be attributed to alternative pathways.
Keywords : metabolism cyanotoxin cylindrospermopsin toxicology HepaRG cell
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Journal articles
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https://hal-anses.archives-ouvertes.fr/anses-01346329
Contributor : Emmanuelle Chiffoleau <>
Submitted on : Monday, July 18, 2016 - 4:59:12 PM
Last modification on : Tuesday, February 19, 2019 - 3:14:07 PM

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K Kittler, Dominique Hurtaud Pessel, R Maul, F Kolrep, Valérie Fessard,. In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions. Toxicon, Elsevier, 2016, 110, pp.47-50. ⟨10.1016/j.toxicon.2015.11.007⟩. ⟨anses-01346329⟩

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