In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions

K Kittler , Dominique Hurtaud Pessel (1) , R Maul , F Kolrep , Valérie Fessard, (1)

1 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail

No evidence for phase I metabolites of the cyanotoxin cylindrospermopsin (CYN) was given using HepaRG cells and different liver tissue fractions when studying metabolic conversion. Although the application of ketoconazole, a CYP3A4 inhibitor, led to a decreased cytotoxicity of CYN, no metabolites were detected applying high resolution mass spectrometry. Quantification of non-modified CYN led to recovery rates of almost 100%. Consequently, reduction of CYN toxicity in the presence of metabolism inhibiting agents must be attributed to alternative pathways.

Keywords: metabolism; cyanotoxin; cylindrospermopsin; toxicology; HepaRG cell

Keywords: métabolisme; cyantoxine; cylindrospermopsine; toxicologie

Domains: Toxicology

K Kittler, Dominique Hurtaud Pessel, R Maul, F Kolrep, Valérie Fessard,. In vitro metabolism of the cyanotoxin cylindrospermopsin in HepaRG cells and liver tissue fractions. Toxicon, 2016, 110, pp.47-50. ⟨10.1016/j.toxicon.2015.11.007⟩. ⟨anses-01346329⟩