Combined Effects of Lipophilic Phycotoxins (Okadaic Acid, Azapsiracid-1 and Yessotoxin) on Human Intestinal Cells Models

Pierre-Jean Ferron, 1 Kevin Dumazeau, 1 Jean-François Beaulieu, 2 Ludovic Le Hegarat, 1 Valerie Fessard, 1
1 Anses Laboratoire de Fougères
2 University of Sherbrooke [Sherbrooke]
Abstract : Phycotoxins are monitored in seafood because they can cause food poisonings in humans. Phycotoxins do not only occur singly but also as mixtures in shellfish. The aim of this study was to evaluate the in vitro toxic interactions of binary combinations of three lipophilic phycotoxins commonly found in Europe (okadaic acid (OA), yessotoxin (YTX) and azaspiracid-1 (AZA-1)) using the neutral red uptake assay on two human intestinal cell models, Caco-2 and the human intestinal epithelial crypt-like cells (HIEC). Based on the cytotoxicity of individual toxins, we studied the interactions between toxins in binary mixtures using the combination index-isobologram equation, a method widely used in pharmacology to study drug interactions. This method quantitatively classifies interactions between toxins in mixtures as synergistic, additive or antagonistic. AZA-1/OA, and YTX/OA mixtures showed increasing antagonism with increasing toxin concentrations. In contrast, the AZA-1/YTX mixture showed increasing synergism with increasing concentrations, especially for mixtures with high YTX concentrations. These results highlight the hazard potency of AZA-1/YTX mixtures with regard to seafood intoxication.
Mots-clés : phycotoxine toxicité toxicologie model
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [43 références]  Voir  Masquer  Télécharger
https://hal-anses.archives-ouvertes.fr/anses-01333030
Contributeur : Emmanuelle Chiffoleau <>
Soumis le : jeudi 16 juin 2016 - 17:10:28
Dernière modification le : mercredi 29 novembre 2017 - 16:02:32

Fichier

toxins-08-00050.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

ANSES

Citation

Pierre-Jean Ferron,, Kevin Dumazeau,, Jean-François Beaulieu,, Ludovic Le Hegarat,, Valerie Fessard,. Combined Effects of Lipophilic Phycotoxins (Okadaic Acid, Azapsiracid-1 and Yessotoxin) on Human Intestinal Cells Models. Toxins, MDPI, 2016, 8 (2), pp.50-63. 〈http://www.mdpi.com/2072-6651/8/2/50〉. 〈10.3390/toxins8020050〉. 〈anses-01333030〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

54

Téléchargements de fichiers

56

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD