Skip to Main content Skip to Navigation
Journal articles

High prevalence of small intestine bacteria overgrowth and asymptomatic carriage of enteric pathogens in stunted children in Antananarivo, Madagascar

Abstract : Environmental Enteric Dysfunction (EED) refers to an incompletely defined syndrome of inflammation, reduced absorptive capacity, and reduced barrier function in the small intestine. It is widespread among children and adults in low- and middle-income countries and is also associated with poor sanitation and certain gut infections possibly resulting in an abnormal gut microbiota, small intestinal bacterial overgrowth (SIBO) and stunting. We investigated bacterial pathogen exposure in stunted and non-stunted children in Antananarivo, Madagascar by collecting fecal samples from 464 children (96 severely stunted, 104 moderately stunted and 264 non-stunted) and the prevalence of SIBO in 109 duodenal aspirates from stunted children (61 from severely stunted and 48 from moderately stunted children). SIBO assessed by both aerobic and anaerobic plating techniques was very high: 85.3% when selecting a threshold of ≥10 5 CFU/ml of bacteria in the upper intestinal aspirates. Moreover, 58.7% of the children showed more than 10 6 bacteria/ml in these aspirates. The most prevalent cultivated genera recovered were Streptococcus , Neisseria , Staphylococcus , Rothia , Haemophilus , Pantoea and Branhamella . Feces screening by qPCR showed a high prevalence of bacterial enteropathogens, especially those categorized as being enteroinvasive or causing mucosal disruption, such as Shigella spp., enterotoxigenic Escherichia coli , enteropathogenic E . coli and enteroaggregative E . coli . These pathogens were detected at a similar rate in stunted children and controls, all showing no sign of severe diarrhea the day of inclusion but both living in a highly contaminated environment (slum-dwelling). Interestingly Shigella spp. was the most prevalent enteropathogen found in this study (83.3%) without overrepresentation in stunted children.
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03677601
Contributor : Floran Bidault Connect in order to contact the contributor
Submitted on : Tuesday, May 24, 2022 - 5:25:18 PM
Last modification on : Monday, May 30, 2022 - 11:40:45 AM
Long-term archiving on: : Tuesday, August 30, 2022 - 10:17:55 AM

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Jean-Marc Collard, Lova Andrianonimiadana, Azimdine Habib, Maheninasy Rakotondrainipiana, Prisca Andriantsalama, et al.. High prevalence of small intestine bacteria overgrowth and asymptomatic carriage of enteric pathogens in stunted children in Antananarivo, Madagascar. PLoS Neglected Tropical Diseases, Public Library of Science, 2022, 16 (5), pp.e0009849. ⟨10.1371/journal.pntd.0009849⟩. ⟨pasteur-03677601⟩

Share

Metrics

Record views

14

Files downloads

5