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A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19

Abstract : This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-expressing HEK293T cells that mimics membrane insertion and N-glycosylation of viral integral membrane proteins in host cells. This assay represents a powerful tool to test antibody responses against SARS-CoV-2 variants and vaccine effectiveness. For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).
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https://hal.archives-ouvertes.fr/hal-03632340
Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Tuesday, May 31, 2022 - 3:01:27 PM
Last modification on : Tuesday, May 31, 2022 - 3:01:28 PM

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Sophie Martin, Gwénaële Jégou, Aurore Nicolas, Matthieu Le Gallo, Éric Chevet, et al.. A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19. STAR Protocols, Elsevier, 2022, 3 (1), pp.101229. ⟨10.1016/j.xpro.2022.101229⟩. ⟨hal-03632340⟩

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