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Article Dans Une Revue STAR Protocols Année : 2022

A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19

Résumé

This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-expressing HEK293T cells that mimics membrane insertion and N-glycosylation of viral integral membrane proteins in host cells. This assay represents a powerful tool to test antibody responses against SARS-CoV-2 variants and vaccine effectiveness. For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).
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hal-03632340 , version 1 (31-05-2022)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Sophie Martin, Gwénaële Jégou, Aurore Nicolas, Matthieu Le Gallo, Éric Chevet, et al.. A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19. STAR Protocols, 2022, 3 (1), pp.101229. ⟨10.1016/j.xpro.2022.101229⟩. ⟨hal-03632340⟩
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